A kind of fermented meat sauce and preparation method thereof
A meat sauce, raw meat technology, applied in biochemical equipment and methods, microorganism-based methods, microorganisms, etc., can solve the problems of hygienic safety and chemical safety to be guaranteed, and achieve the purpose of reducing accumulation, improving digestibility, The effect of improving food safety
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Embodiment 1
[0032] Example 1 Isolation and identification of Pediococcus pentosaceus 37x-15
[0033] (1) Strain isolation
[0034] Accurately weigh 25g of air-dried beef in the ultra-clean bench, cut it into pieces, and place it in 225 mL of sterilized normal saline, that is, the dilution is 10 -1 the sample solution, continue to dilute the sample solution to 10 -2 , 10 -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 , respectively draw 10 well-mixed -3 , 10 -4 , 10 -5 , 10 -6 , 10 -7 100uL of the diluent was evenly spread on MRS+0.7% calcium carbonate solid medium, cultured anaerobic at 30°C for 48h, and colonies with obvious calcium circles were picked and streaked repeatedly until pure strains were obtained. The Gram-positive and catalase-negative strains were tentatively designated as lactic acid bacteria. Add 30% glycerol to the culture medium of all pure strains, and store them frozen at -80°C.
[0035] (2) Strain activation and purification
[0036]Take out the preserved strain, p...
Embodiment 2
[0045] The present embodiment specifically provides a preparation method of fermented meat sauce, which specifically includes the following content:
[0046] 1. Raw Meat Fermentation
[0047] (1) Processing of meat: Wash fresh meat or thawed raw meat with water to remove blood, and then use a meat grinder to mince it for later use.
[0048] (2) Washing bacteria: Take 10 mL of Pediococcus pentosaceus 37x-15 and the third generation of Staphylococcus carves into a refrigerated high-speed centrifuge, centrifuge at 3500 r / min for 10 min, remove the supernatant after taking out, and add 10 mL of the concentration to 0.85% After mixing, centrifuge again according to the above conditions, remove the supernatant after taking it out, add 10 mL of physiological saline with a concentration of 0.85%, and shake well for use.
[0049] (3) Fermentation of raw meat: in raw meat according to 10 8 The inoculum amount of cfu / g was inserted into the composite starter (Peococcus pentosaceus 37x-...
Embodiment 3
[0061] Embodiment 3 Determination of growth characteristics of composite starter
[0062] (1) Strain antagonism experiment
[0063] The antagonism experiments of the strains were carried out on the solid medium simulating broth medium. The activated three-generation Staphylococcus aureus and Pediococcus pentosaceus 37x-15 were cross-striated, and after culturing at 30°C for 48 hours, the growth of the strains at the crossing points was observed. Have figure 2 It can be seen that both strains grow well, and there is no mutual inhibition phenomenon. It shows that there is no antagonism between the above two strains.
[0064] (2) Strain growth curve experiment
[0065] Pediococcus pentosaceus 37x-15 was activated in MRS liquid medium for 2 passages, Staphylococcus carnosus was activated in TPY liquid medium for 2 passages, Pediococcus pentosaceus 37x-15 and Staphylococcus cariosus were activated according to 1: 1, 1: 2, 2: The ratio of 1 was inoculated with 1% in the simulat...
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