Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Culture medium of germination of orchid seed

A germination medium and medium technology, applied in the field of orchid seed germination medium, can solve the problems of low germination rate, inability to meet the requirements of introduction cultivation and large-scale planting, and long germination cycle of orchid seeds, and achieve low cost and shortened seed production. Germination time, effect of improving seed germination rate

Inactive Publication Date: 2009-01-28
INST OF BOTANY CHINESE ACAD OF SCI
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the current method, the germination period of orchid seeds is long and the germination rate is not high, which cannot meet the needs of introduction cultivation and large-scale planting.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Culture medium of germination of orchid seed
  • Culture medium of germination of orchid seed
  • Culture medium of germination of orchid seed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Medium I was used to cultivate the seeds of Doritis pulcherrima Lindl. and Vanda coerulescens Griff.

[0021] 1. Experimental materials:

[0022] Seeds: In this experiment, the seeds of Wuliulan and Xiaolan Vanda were used as experimental materials. The mature pods of Wuliulan and Xiaolan Vanda were collected from Hainan.

[0023] Medium: The composition of medium I is shown in Table 1.

[0024] Table 1. Non-symbiotic germination high-efficiency medium I

[0025]

[0026] The preparation method of mashed potatoes is as follows: wash the potatoes, peel them, cut them into small pieces with a knife, and then mash them in a tissue masher, and the obtained homogenate is the mashed potatoes.

[0027] 2. The method of cultivating seeds with this medium I is as follows:

[0028] 1. Seed disinfection:

[0029] (1) Disinfectant treatment: take the fully mature fruit pods, open the peel to take out the seeds, put the seeds in a 10.0ml centrifuge tube, and then add disinfe...

Embodiment 2

[0036] Example 2. Cultivation of the seeds of Phalaenopsis and Vanda blue with medium II.

[0037] 1. Experimental materials:

[0038] Seeds: In this experiment, the seeds of Wuliulan and Xiaolan Vanda were used as experimental materials. The mature fruit pods of Wuliulan and Xiaolan Vanda were collected from Hainan.

[0039] Medium: The composition of medium II is shown in Table 2.

[0040] Table 2. High-efficiency medium for non-symbiotic germination of epiphytic orchid II

[0041]

[0042] Wherein, the preparation method of mashed potatoes is as described in Example 1.

[0043] 2. The method of cultivating seeds with this medium II is as follows:

[0044] 1. Seed disinfection: The method is as described in Example 1.

[0045] 2. Cultivation:

[0046] According to the method described in Example 1, the seeds sterilized in step 1 were inoculated into medium II.

[0047] The culture conditions are: dark culture for 2 weeks at a temperature of 25°C and a relative humidity of 50%....

Embodiment 3

[0050] Example 3 Cultivation of the seeds of Phalaenopsis and Vanda blue with medium III.

[0051] 1. Experimental materials:

[0052] Seeds: In this experiment, the seeds of Wuliulan and Xiaolan Vanda were used as experimental materials. The mature pods of Wuliulan and Xiaolan Vanda were collected from Hainan.

[0053] Medium: The composition of medium III is shown in Table 3.

[0054] Table 3. High-efficiency medium for non-symbiotic germination of epiphytic orchid III

[0055]

[0056] Wherein, the preparation method of mashed potatoes is as described in Example 1.

[0057] 2. The method of cultivating seeds with this medium III is as follows:

[0058] 1. Seed disinfection: The method is as described in Example 1.

[0059] 2. Cultivation:

[0060] According to the method described in Example 1, the seeds sterilized in step 1 were inoculated into medium III.

[0061] The culture conditions are: dark culture for 2 weeks at a temperature of 24°C and a relative humidity of 49%. A...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a medium for the seed germination of orchids; the medium consists of NH4NO3, KNO3, CaCl2.2H2O, MgSO4.7H2O, KH2PO4, KI, H3BO3, MnSO4.4H2O, ZnSO4.7H2O, Na2MoO4.2H2O, CuSO4.5H2O, CoCl2.6H2O, FeSO4.7H2O, Na2.EDTA, inosite, nicotinic acid, pyridoxine hydrochloride, thiamine hydrochloride, glycine, peptone from casein, mashed potato and sucrose and the volume is limited by water; by using the medium of the invention to culture orchid, not only the seed germination time can be shortened but also the seed germination rate can be improved; the experiment indicates that by using the medium of the invention to culture orchid, only two weeks are needed from seeding to germination and the germination rate is more than 70 percent. The method of the invention is suitable for culturing various caulicolous orchids and has the advantages of simple operation and low cost; therefore, the medium and the method for culturing the orchids of the invention have important promotion value and are applicable to practice use.

Description

Technical field [0001] The invention relates to an orchid seed germination culture medium. Background technique [0002] There are about 775 genera and 19,500 species of orchids. They are widely distributed in tropical, subtropical and temperate zones. They are mainly produced in South America and tropical Asia. There are more than 1,000 species of 166 genera in my country, which are mainly distributed in Jiangnan provinces, with Guangdong, Hainan, Yunnan and Taiwan in the majority. Orchid plants are a special plant group and one of the most evolved families in angiosperms; among them, Bletilla striata and Gastrodia can be used for medicinal purposes; a few can be used as spice plants, such as vanilla, etc.; and most of them have bright colors and long flowering periods , Can be used as a valuable ornamental plant, with high commercial value. [0003] Among the 1300 species of endangered plants listed in my country's key protection, orchids account for more than 1,200 species, ac...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01C1/00A01G31/00
Inventor 王瑞霞宋松泉何明高宋希强
Owner INST OF BOTANY CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com