Culture medium of germination of orchid seed
A germination medium and medium technology, applied in the field of orchid seed germination medium, can solve the problems of low germination rate, inability to meet the requirements of introduction cultivation and large-scale planting, and long germination cycle of orchid seeds, and achieve low cost and shortened seed production. Germination time, effect of improving seed germination rate
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Embodiment 1
[0020] Example 1. Medium I was used to cultivate the seeds of Doritis pulcherrima Lindl. and Vanda coerulescens Griff.
[0021] 1. Experimental materials:
[0022] Seeds: In this experiment, the seeds of Wuliulan and Xiaolan Vanda were used as experimental materials. The mature pods of Wuliulan and Xiaolan Vanda were collected from Hainan.
[0023] Medium: The composition of medium I is shown in Table 1.
[0024] Table 1. Non-symbiotic germination high-efficiency medium I
[0025]
[0026] The preparation method of mashed potatoes is as follows: wash the potatoes, peel them, cut them into small pieces with a knife, and then mash them in a tissue masher, and the obtained homogenate is the mashed potatoes.
[0027] 2. The method of cultivating seeds with this medium I is as follows:
[0028] 1. Seed disinfection:
[0029] (1) Disinfectant treatment: take the fully mature fruit pods, open the peel to take out the seeds, put the seeds in a 10.0ml centrifuge tube, and then add disinfe...
Embodiment 2
[0036] Example 2. Cultivation of the seeds of Phalaenopsis and Vanda blue with medium II.
[0037] 1. Experimental materials:
[0038] Seeds: In this experiment, the seeds of Wuliulan and Xiaolan Vanda were used as experimental materials. The mature fruit pods of Wuliulan and Xiaolan Vanda were collected from Hainan.
[0039] Medium: The composition of medium II is shown in Table 2.
[0040] Table 2. High-efficiency medium for non-symbiotic germination of epiphytic orchid II
[0041]
[0042] Wherein, the preparation method of mashed potatoes is as described in Example 1.
[0043] 2. The method of cultivating seeds with this medium II is as follows:
[0044] 1. Seed disinfection: The method is as described in Example 1.
[0045] 2. Cultivation:
[0046] According to the method described in Example 1, the seeds sterilized in step 1 were inoculated into medium II.
[0047] The culture conditions are: dark culture for 2 weeks at a temperature of 25°C and a relative humidity of 50%....
Embodiment 3
[0050] Example 3 Cultivation of the seeds of Phalaenopsis and Vanda blue with medium III.
[0051] 1. Experimental materials:
[0052] Seeds: In this experiment, the seeds of Wuliulan and Xiaolan Vanda were used as experimental materials. The mature pods of Wuliulan and Xiaolan Vanda were collected from Hainan.
[0053] Medium: The composition of medium III is shown in Table 3.
[0054] Table 3. High-efficiency medium for non-symbiotic germination of epiphytic orchid III
[0055]
[0056] Wherein, the preparation method of mashed potatoes is as described in Example 1.
[0057] 2. The method of cultivating seeds with this medium III is as follows:
[0058] 1. Seed disinfection: The method is as described in Example 1.
[0059] 2. Cultivation:
[0060] According to the method described in Example 1, the seeds sterilized in step 1 were inoculated into medium III.
[0061] The culture conditions are: dark culture for 2 weeks at a temperature of 24°C and a relative humidity of 49%. A...
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