Culture medium for spore germination and seedling culturing of pteridophyte

A technology for spore germination and ferns, which is applied in the field of culture medium in the process of industrialized seedling cultivation, can solve the problems of limiting the development, utilization and scientific research of ferns, and low spore germination rate

Inactive Publication Date: 2010-12-15
CHINA UNIV OF GEOSCIENCES (WUHAN)
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Under natural conditions, the spore germination rate is extremely low, th

Method used

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Embodiment 1～ Embodiment 7

[0033] A culture medium suitable for fern spore germination and seedling cultivation, comprising the following nutrient components, see Table 1.

[0034] Table 1: (except for the pH value, the units of the other ingredients are mmol·L -1 )

[0035] Element

[0036] Na 2 MoO 4 2H 2 o

[0037] Specific configuration method: Add the above ingredients to 1L of water to adjust the pH value. Sucrose or agar can be added according to the research purpose. If it is for aseptic germination, the prepared medium needs to be sterilized by moist heat at 121°C for 20 minutes. Among the above-mentioned embodiments, embodiments 1-4 are suitable for the germination of most fern spores. Embodiment 5 is suitable for the germination of fern spores in some basophilic environments. Embodiment 6 is then suitable for the germination of the spores of some salt-tolerant ferns. Embodiment 7 is suitable for the germination of some fern spores that are more sensitive to salt...

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Abstract

The invention belongs to the technical field of biological engineering, and relates to a culture medium for the spore germination and seedling culturing of pteridophyte, which is characterized by consisting of the following nutritional components: 2 to 60 mmol.L<-1> nitrate ions, 1 to 30 mmol.L<-1> ammonium ions, 0.3 to 8 mmol.L<-1> phosphate radical ions, 2.7 to 66 mmol.L<-1> potassium ions, 0.37 to 12.0 mmol.L<-1> calcium ions, 0.18 to 1.8 mmol.L<-1> magnesium ions, 0.173 to 1.73 mmol.L<-1> sulfate ions, 10 to 100 mumol.L<-1> chelated ferric salt or chelated ferrous salt, 10 to 200 mumol.L<-1> boric acid, 10 to 100 mumol.L<-1> divalent manganese ions, 3 to 30 mumol.L<-1> zinc ions, 0.5 to 8 mumol.L<-1> divalent copper ions or complexes thereof, 0.1 to 1 mumol.L<-1> molybdate ions, 0.01 to 0.1 mumol.L<-1> divalent cobalt ions, 0.05 to 12 mumol.L<-1> chloride ions, 0.3 to 30 mumol.L<-1> thiamine hydrochloride and the balance of water, wherein the pH value of the culture medium is between 4.0 and 9.0. The culture medium can improve the germination rate of spores.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a culture medium in the process of industrialized seedling cultivation of ferns. Background technique [0002] Ferns, also known as ferns, have independent living gametophytes (protophylls) and sporophytes, and their sporophytes have vessels, which are lower vascular plants. my country is rich in fern resources and is known as the "Kingdom of Ferns". Ferns are of great significance in many aspects such as ecosystems, bioremediation of polluted sites, social economy, and scientific research. Therefore, the present invention has wide application prospect, now exemplifies as follows: [0003] Many ferns have edible value, some are well-known mountain treasures, such as Osmunda (including Osmunda japonicThunb. and Osmunda cinnamomea L.), bracken, pod fern [Matteuccia struthiopteris (L.) Todaro, Also known as cucumber fragrance, Cantonese cuisine], monkey leg (sci...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 葛台明郑敏李继红王焰新
Owner CHINA UNIV OF GEOSCIENCES (WUHAN)
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