Method for forcing dunaliella tertiolecta to accumulate beta-carotene

A technology of Dunaliella salina and carotene, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of high cost, slow growth, poor product quality, etc.

Active Publication Date: 2015-03-25
临沂东旭食品有限公司
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Problems solved by technology

[0005] Traditionally, the large-scale cultivation of Dunaliella salina adopts open raceway-type large ponds and uses Hutner culture medium for cultivation. The light intensity and temperature are completely dependent on nature, which is erratic and...

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  • Method for forcing dunaliella tertiolecta to accumulate beta-carotene

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Embodiment Construction

[0015] ① Preparation of step 1 culture medium Composition of No. 1 culture medium: 1000 parts of boiled and cooled seawater, 15 parts of sodium chloride, 2 parts of sodium nitrate, 1 part of ammonium chloride, 1.5 parts of human urine, 0.04 parts of calcium phosphate, ammonium sulfate 0.5 parts, 0.002 parts of ferric chloride, 0.001 parts of thiamine hydrochloride, 0.002 parts of niacin, vitamin B 12 0.001 part; take 2000 ml of this culture solution and put it into a 3000 ml Erlenmeyer flask, seal it with plastic wrap and a rubber band, shake it on a shaker for 15 minutes, and put it in a refrigerator at 4°C for future use. Ingredients of No. 2 culture medium: 1000 parts of boiled and cooled seawater, 25 parts of sodium chloride, 0.002 part of ferric chloride, 0.001 part of thiamine hydrochloride, 0.002 part of niacin, vitamin B 12 0.001 part; after shaking well, put it in a refrigerator at 4°C and store it for later use.

[0016] ② Step 2 inoculation culture Take a 3000 ml c...

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Abstract

The invention discloses a method for forcing dunaliella tertiolecta to accumulate beta-carotene and belongs to the aquaculture field. Conventionally, the dunaliella tertiolecta adopts an open type runway big pool and uses an Hutner culture solution to culture, is slow in growth, low yin yield, easy to pollute, high in cost and low in content of beta-carotene, and the illumination intensity and the temperature are completely dependent on nature. In order to overcome the defects in the prior art, indoor light-control temperature-control culture is adopted, the illumination intensity and the temperature are regulated according to the special needs of different growth stages of the dunaliella tertiolecta, especially at the later culture period, the illumination intensity and the temperature are increased, ammonium chloride, human urine, calcium phosphate, thiamine hydrochloride and the like are supplemented, the conventional bath culture method is changed; after the later culture period, nitrogen salt and phosphor salt in the culture solution are removed, the salinity is improved, the growth speed of the dunaliella tertiolecta is greatly increased, and the content of the beta-carotene is increased by 70%.

Description

technical field [0001] The invention belongs to the field of aquaculture, and in particular relates to a method for stressing Dunaliella salina to accumulate beta-carotene. Background technique [0002] Dunaliella salina is a diflagellated unicellular green alga that can tolerate high salinity (20-300‰). Dunaliella salina can accumulate a large amount of β-carotene under stress conditions, up to 14% of dry weight, which is the first of all organisms in nature. Therefore, as early as 1966, Massyuk proposed that Dunaliella salina can be cultivated on a large scale to produce β-carotene; in addition, the glycerol contained in Dunaliella salina can reach 40% of the dry weight, which has the potential for producing glycerol; Dunaliella salina is also a good feed for aquatic animals. my country has a long coastline and numerous inland salt lakes, and has unique natural conditions for cultivating Dunaliella salina to produce β-carotene. [0003] The types and proportions of nitro...

Claims

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Application Information

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IPC IPC(8): C12P23/00C12R1/89
Inventor 王培磊
Owner 临沂东旭食品有限公司
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