Large-scale sympodial bamboo callus induction and plant regeneration high efficiency culture medium and culture method thereof

A technology of callus induction and culture medium, applied in plant regeneration, botany equipment and methods, horticultural methods, etc., can solve the problems of difficult cultivation of plant regeneration, induction of callus of large clustered bamboo, etc., and achieve accelerated differentiation and rooting Effect

Inactive Publication Date: 2010-10-06
胡尚连
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  • Summary
  • Abstract
  • Description
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Problems solved by technology

[0002] At present, there are many applications of bamboo at home and abroad, especially the large-scale clustered bamboo used for pulp, so the demand for bamboo is relatively large, but there are problems when cultivating large-scale clustered bamboo, especially bamboo for pulp. Ti

Method used

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Embodiment

[0044] High-efficiency medium for callus induction and plant regeneration of large cluster bamboo, including MS medium and high-efficiency medium for callus induction and plant regeneration. MS medium is made according to the following raw materials:

[0045] a) Major elements (mg / L): potassium nitrate (KNO3) 1900, ammonium nitrate (NH4NO3) 1650, potassium dihydrogen phosphate (KH2PO4) 170, magnesium sulfate (MgSO4.7H2O) 370, calcium chloride (CaCl2.2H2O) 440

[0046] b) Trace elements (mg / L): potassium iodide (KI) 0.83, boric acid (H3BO3) 6.2, manganese sulfate (MnSO4.4H2O) 22.3, zinc sulfate (ZnSO4.7 H2O) 8.6, sodium molybdate (Na2MoO4.2 H2O) 0.25, copper sulfate (CuSO4.5 H2O) 0.025, cobalt chloride (CoCl2) 0.025

[0047] c) Organic ingredients (mg / L): inositol 100, glycine 2, thiamine hydrochloride (VB1) 0.1, pyridoxine hydrochloride (VB6) 0.5, niacin (VB5 or VPP) 0.5

[0048] d) Iron salt (mg / L): disodium ethylenediaminetetraacetic acid (Na2.EDTA) 37.3, ferrous sulfate (...

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Abstract

The invention relates to a culture medium suitable for a large-scale sympodial bamboo including sinocalamus affinis and Mianzhu and a culture method, in particular to a large-scale sympodial bamboo callus induction and plant regeneration high efficiency culture medium and a culture method thereof. The culture medium comprises an MS (Murashige and Skoog) culture medium and a callus induction and plant regeneration high efficiency culture medium and is characterized in that the MS culture medium is prepared from the following raw materials: (a) macroelements: potassium nitrate, ammonium nitrate, potassium dihydrogen phosphate, magnesium sulfate and calcium chloride; (b) microelements: potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulfate and cobalt chloride; (c) organic components: inosite, glycine, aneurin hydrochloride, puridoxinehydrochloride and niacin; and (d), ferric salts: disodiumedetate and ferrous sulfate.

Description

technical field [0001] The invention relates to a culture medium and a culture method suitable for large-scale clumping bamboos such as Cizhu and Mianzhu, in particular to a high-efficiency medium for callus induction and plant regeneration of large-scale clumping bamboos and a cultivation method thereof. Background technique [0002] At present, there are many applications of bamboo at home and abroad, especially large-scale clustered bamboos for pulp, so the demand for bamboo is relatively large, but there are problems when cultivating large-scale clustered bamboos, especially bamboos for pulp. Tissue culture studies mostly focus on the rapid propagation of ornamental bamboos. The callus induction and plant regeneration of large clustered bamboos, especially pulp bamboos, are difficult to cultivate, and there are no reports on the relevant materials at home and abroad. Contents of the invention [0003] The present invention is aimed at the above technical problems, and ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 胡尚连曹颖陈珂周建英段宁卢学琴
Owner 胡尚连
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