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Oryza meyeriana in vitro immature embryo medium

A technology of wild rice verrucas and a culture medium, applied in the field of culture medium, can solve the problems of low nutrient content, low seedling emergence rate, hinder the utilization of excellent germplasm of wild rice verrucas, and achieve high survival rate of young embryos and high seedling emergence rate. Effect

Inactive Publication Date: 2016-03-16
广西壮族自治区农业科学院水稻研究所
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Problems solved by technology

Oryza verruca is one of the three existing wild rices in my past. It has high resistance to bacterial blight and is close to immunity, and its resistance is long-lasting. It is an ideal breeding material for disease resistance; When cultured in medium, the lack of certain nutrients or low nutrient content in the medium will cause the problem of low emergence rate
It is also very difficult to obtain the seedlings of Oryza sativa by other germination methods, and its germination rate is extremely low, which seriously hinders the utilization of the excellent germplasm of Oryza sativa

Method used

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  • Oryza meyeriana in vitro immature embryo medium
  • Oryza meyeriana in vitro immature embryo medium
  • Oryza meyeriana in vitro immature embryo medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A culture medium for isolated immature embryos of wild rice verruca, each liter of culture medium includes:

[0017]

[0018]

Embodiment 2

[0020] A culture medium for isolated immature embryos of wild rice verruca, each liter of culture medium includes:

[0021]

[0022]

Embodiment 3

[0024] A culture medium for isolated immature embryos of wild rice verruca, each liter of culture medium includes:

[0025]

[0026]

[0027] The cultivation effect of the culture medium provided by the invention is higher than that of MS, N6, He V, L8, White, Miller, Nitsch., T, H, B5 and other culture media, and the seedling emergence rate is increased by about 15%.

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Abstract

An Oryza meyeriana in vitro immature embryo medium contains KNO2, (NH4)3SO4, KH2PO4, MgSO4.7H2O, CaCl2.2H2O, MnSO4.7H2O, ZnSO4.7H2O, H3BO4, KI, NaMoO4.2H2O, CuSO4.5H2O, CoCl.6H2O, glycine, thiamine hydrochloride, pyridoxol hydrochloride, nicotinic acid, inositol, lactoalbumin hydrolysate, a ferric salt, sucrose, 6-BA, IAA and agar. Above components include various nutritional components and contents needed in the Oryza meyeriana in vitro immature embryo culture process, so the medium has the advantages of high emergence rate and high survival rate of immature embryos.

Description

【Technical field】 [0001] The invention relates to a culture medium, in particular to a culture medium for isolated immature embryos of Oryza verruca. 【Background technique】 [0002] Rice bacterial blight is one of the three major diseases threatening rice production in my country. Breeding and application of rice varieties resistant to bacterial blight is the most economical and effective control method. Rice disease-resistant varieties in my country have a single source of resistance, and large-scale planting of single-resistant varieties has the risk of causing a pandemic. Therefore, it is very important to find new sources of resistance. Oryza verruca is one of the three existing wild rices in my past. It has high resistance to bacterial blight and is close to immunity, and its resistance is long-lasting. It is an ideal breeding material for disease resistance; When cultured in medium, due to the lack of certain nutrients or low nutrient content in the medium will cause ...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 赖群珍陈成斌梁云涛张烨梁世春潘英华
Owner 广西壮族自治区农业科学院水稻研究所
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