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Method for breeding autoallopolyploid rice

An allopolyploid, rice technology, applied in rice cultivation, microbial determination/inspection, introduction of foreign genetic material using vectors, etc. stability and other issues, to achieve the effect of large grain size and improved seed setting rate

Active Publication Date: 2007-10-03
WUHAN POLYPLOID BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After analysis, the reason for the low seed setting rate of polyploid rice is mainly that the genetic relationship is too close and lacks meiotic stability, so the polyploid meiotic stability (PMeS) strain (Cai DT et al, Breeding of polyploidy rice) was selected and bred. pines with polyploidy meiosis stability, Chinese Science.C series Life Science, 2007.1: 1-9) solved the problem of low seed-setting rate of polyploid hybrids between indica and japonica subspecies, and constructed a heterologous Tetraploid rice (AABB and AAEE) hybrids, but prone to seed shattering, less than 70% seed setting rate, and smaller grains than indica-japonica tetraploid hybrids, which are only comparable to diploid cultivated rice

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: Breeding of autologous allohexaploid rice DHI238

[0035] a. Select parent

[0036] Allotetraploid (A s A s BB) Cw008-4x was used as the female parent, and PMeS strain HN2026-2x was used as the male parent to select 5 strains each.

[0037] b. Hybridization and Hormone Treatment

[0038] With Cw008-4x as the female parent, suitable rice ears were selected at 7:30 in the morning at the peak flowering stage, and 1 / 3 of the glumes were cut off, anthers were removed with tweezers, and bagged. Then spray hormone mixture (2,4-D+GA 3 ), let it dry slightly, take the ear of HN2026-2X that is about to bloom as the male parent at about 10:30, put it on the hybridization bag of the detasselled rice ear, and shake it gently with your hands when the spikelets of the male parent are open, so that the pollen can be as much as possible. Scattered on the stigma of the female parent in many places, usually provided by the male parent of 3 spikes, fold the mouth of the ...

Embodiment 2

[0056] Example 2. Breeding of homologous and heterologous octoploid rice DHI237

[0057] a. Select parent

[0058] With allotetraploid Cy301-4x(A s A s BB) was one of the parents, and the PMeS line Sg99012-4x was used as the other parent to select 5 strains each.

[0059] b. Hybridization and Hormone Treatment

[0060] With Cy301-4x as the female parent and Sg99012-4x as the male parent, the method of Example 1 was used for hybridization and hormone treatment.

[0061] c. Embryo rescue culture

[0062] Using the same method as in Example 1, 28 young ovaries were inoculated to form 10 calli, and the induction rate was 35.7%.

[0063] d. Chromosome doubling

[0064] 10 pieces of callus were transferred into the callus doubling liquid, and cultured according to the method of Example 1.

[0065] e. Double callus differentiation and emergence

[0066] 10 calli were cultivated and differentiated into 5 shoots under the same differentiation conditions as in Example 1, and the...

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PUM

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Abstract

A method for selectively culturing the irregular allopolyploid rice includes such steps as choosing parents, hybridizing while treating with hormone, embryonic retrieval culturing, reduplicating chromosome, reduplicating calli or fasciculate embryos, growing to become seedlings, rooting culture, transplanting, culturing and discriminating. The resultant rice may be irregular allohexaploid rice or irregular allooctaploid rice.

Description

technical field [0001] The invention relates to the selection and breeding of homologous allopolyploid rice and belongs to the technical field of polyploid rice breeding. Background technique [0002] At present, rice breeding in the world, including conventional breeding, hybrid breeding and molecular breeding, is all based on the diploid level. Limited by sexual reproduction, more than 20 favorable genes in wild rice cannot be widely used, and it is difficult to increase yield by more than 50%. However, polyploidization is an important way of plant evolution in nature. About 70% of higher plants are polyploid, and major grain, cotton and oil crops such as wheat, cotton, rapeseed, etc. are also polyploid, and they have experienced diploid The process of evolution towards polyploidy, accompanied by a doubling of production. Therefore, Cai Detian, Yuan Longping, and Lu Xinggui proposed in Chinese patent ZL00114471.5 the breeding technology of super rice using the dual advant...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H4/00A01H1/06A01H5/00A01G16/00C12N5/04C12Q1/68C12N15/65A01G22/22
Inventor 侯明辉朱姣姣万春春陈冬玲宋兆建蔡得田
Owner WUHAN POLYPLOID BIOTECH CO LTD
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