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Cutting seedling-raising method capable of making oryza plants ward off diseases and detoxified rapidly

A technology for raising seedlings by cuttings and rice genus, which is applied in the directions of botanical equipment and methods, planting substrates, rice cultivation, etc., can solve the problems of re-contamination of endophytic bacteria, large-scale fungal contamination, no public reports, etc., and achieves low cost, Short cycle times and the effect of reducing the risk of contamination

Inactive Publication Date: 2017-07-21
云南省农业科学院生物技术与种质资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the conventional cutting method is prone to large-scale fungal contamination in the early stage of cultivation, and re-contamination of endophytic bacteria sometimes occurs during the cultivation process, resulting in material loss
[0005] The present invention provides a cutting seedling raising method that can quickly remove diseases and viruses from Oryza plants. After literature search, there is no public report on the same technology as the present invention.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1: Yunnan Medicinal Wild Rice Stem Cutting Rejuvenation

[0019] In this example, the medicinal wild rice (Oryza officinalis Wall.) grown in the wild in Menghai County, Xishuangbanna Dai Autonomous Prefecture, Yunnan Province was taken as the experimental material, and a batch of medicinal wild rice with higher survival rate was obtained by cutting by the method of the present invention. seedling. The implementation process is as follows:

[0020] 1) Field materials: The medicinal wild rice plants are collected from the wild in Menghai County, Xishuangbanna Dai Autonomous Prefecture, Yunnan Province. At 7 o'clock in the morning on a sunny day, scissors are used to cut the knotted stems on the thin medicinal wild rice plants. Immediately after soaking in a bucket filled with clean water to avoid dehydration of the stems, and then wrap the stems with plastic wrap according to different strains, put them in ice bags to keep fresh and bring them back to the laborat...

Embodiment 2

[0026] Example 2: Stem cutting and rejuvenation of hybrid offspring of Yunnan medicinal wild rice and cultivated rice

[0027]In this example, in the field of Yuanjiang County, Yuxi City, Yunnan Province, the offspring of the hybridization of Yunnan medicinal wild rice and cultivated rice HY-8 were collected as experimental materials, and a batch of cuttings with high survival rate were obtained by using the method of the present invention. Seedlings of hybrid offspring of medicinal wild rice and cultivated rice.

[0028] The implementation process is basically the same as in Embodiment 1, only the following points are different:

[0029] 1) Stem detoxification, detoxification and sterilization treatment: Rinse the stems of offspring hybrids of Yunnan medicinal wild rice and cultivated rice HY-8 brought back to the laboratory with running tap water for 10 minutes, and thoroughly rinse off the mud and dirt on the surface Finally, wipe the stalks with 75% alcohol cotton balls b...

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PUM

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Abstract

The invention relates to a cutting seedling-raising method capable of making oryza plants ward off diseases and detoxified rapidly and belongs to the technical field of plant detoxification. The cutting seedling-raising method capable of making the oryza plants ward off diseases and detoxified rapidly comprises the steps of material taking form fields, disease warding-off, detoxification and sterilization treatment conducted on stems, stem cuttage preprocessing, stem cuttage and stem rejuvenation and cultivation. By utilizing a plant disease warding-off and detoxification technology, the pollution problem in the oryza stem cuttage process is solved effectively, the stems are higher in survival rate after cuttage, cutting seedlings grow vigorously and are good in growth vigor, and an effective means is provided for rejuvenation and rapid propagation of important hybridization materials and saving of wild rice high-quality germplasm resources. The method is simple, easy to operate and low in cost, the oryza plants surviving through cuttage are good in growth vigor, and diseases and insect pests do not occur basically. In addition, a cutting seedling-raising period is short, next transplantation can be performed in 1-2 months generally and is not affected by seasons and other environmental factors, and large-scale popularization and application can be achieved. The method can be also applied to saving and rapid propagation of certain rare oryza varieties.

Description

technical field [0001] The invention relates to a method for growing seedlings by cuttings capable of quickly detoxifying oryza plants, belonging to the technical field of plant detoxification. Background technique [0002] Oryza genus, Poaceae, about 25 species, distributed in tropical regions, divided into cultivated rice and wild rice. Wild rice is a general term for all wild species other than cultivated rice (O. sativa L.) and African cultivated rice (O. glaberrima Steud.). [0003] Long-term artificial selection has made the genetic background of cultivated rice in my country very narrow. The narrow genetic background, coupled with the growth of global population in recent years, the reduction of cultivated land area, and global climate change, make rice production vulnerable to pests and diseases and environmental pollution. Lead to a serious decline in rice yield and quality. Wild rice has long-term survival in the wild and has strong stress resistance. It is a natu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G16/00A01G9/10A01G31/00A01G31/02A01G22/22
CPCA01G22/22A01G24/00A01G31/00A01G31/02Y02P60/21
Inventor 陈越程在全陈玲王玲仙殷富有柯学肖素勤钟巧芳付坚王波张敦宇余腾琼李定琴蒋聪曾民杨雅云黄兴奇
Owner 云南省农业科学院生物技术与种质资源研究所
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