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Polyploid rice breeding and detecting method with polyploid meiosis stability gene
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A technology of meiosis and polyploidy, applied in the field of polyploid rice breeding and identification, can solve the problem of low seed setting rate of polyploid rice
Active Publication Date: 2005-03-02
WUHAN POLYPLOID BIOTECH CO LTD
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[0005] The purpose of the present invention is to select and breed the polyploid rice of polyploid meiosis stability (PMeS) gene, in order to solve the problem of low seed setting rate of polyploid rice, and the PMeS strain that makes it form is used in polyploid Somatic Rice Breeding
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Embodiment 1
[0034] Embodiment 1: Breeding of PMeS-1 (Sg99012)
[0035] a. Select parent
[0036] Rice roll (indica type), sorghum rice (japonica type) and GC574 (japonica type) were used as parents, and 10 plants were selected each.
[0037] b. Hybridization and compound hybridization
[0038] Curly leaf rice × sorghum rice gets F 1 -a, Curly leaf rice × GC574 get F 1 -b, then F 1 -a and F 1 -b Perform compound crosses. Select 50 hybrids.
[0039] c. Selection of hybrids for continuous backcrossing
[0040] Select 10 hybrid plants of the japonica type, compact plant type, straight flag leaves, large ears, and dense grains, and use GC574 as the female parent to backcross to obtain BC 1 f 1 (47 strains), from which the hybrid strains (30 strains) of the inclined japonica type, compact plant type, straight flag leaves, large ears, and dense grains were selected and backcrossed with GC574 to obtain BC 2 f 1 (15 strains), continuous backcrossing 3 to 4 times to obtain BC 4 f 1 (se...
Embodiment 2
[0053] Embodiment 2: Breeding of PMeS-2 (HN2026)
[0054] a. Select parent
[0055] HT99104 (japonica type) and Shuhui 362 (indica type) were used as parents, and 10 plants were selected each.
[0056] b. Hybridization or compound hybridization
[0057] HT99104×Shuhui 362, obtained 12 F 1 .
[0058] c. Selection of hybrids for continuous backcrossing
[0059] It is basically the same as Example 1, except that HT99104 is used as the backcross parent, and BC is obtained after four times of backcross 4 f 1 15 hybrids.
[0061] The young paniclecallus was used, induced to double in N6 liquid medium supplemented with 500 mg / l colchicine, and then differentiated into polyploid rice seedlings.
[0062] E, f, g process are substantially the same as embodiment 1.
[0063] h. Self-bred stable product line
[0064] The polyploid rice single plant selected in the g process was selfed to stabilize it into a line, and the line was propagated into a...
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Abstract
The present invention provides polyploid rice selectively breeding and identifying method with polyploidmeiosis stability (PMeS) gene. The rice selectively breeding process includes: selecting parents; hybridization or complex hybridization; back crossing; doubling chromosome; identifying polyploid; selecting bearing property; observing meiosis behavior; self-crossing to form stable line; and identifying high bearing property. The identifying indexes include: polyploid line of Asia cultivated indica type rice or japonica rice; bearing rate not lower than 65 %; normal meiosis behavior; normal pollen shape and physiological activity and normal dyed pollution with 0.1 5 I-KI not lower than 95 %; bearing rate of hybrid of PMeS polyploid rice and other polyploid rice variety over 70 % and that of PMeS polyploid rice and wild rice variety over 65 %. The present invention solves the problem of low bearing rate of polyploid rice and may be used in breeding polyploid rice variety.
Description
technical field [0001] The invention relates to a polyploid rice breeding and detection method with a polyploid meiosis stability (Polyploid MeiosisStability, PMeS) gene, belonging to polyploid rice breeding [0002] technology field. Background technique [0003] About 70% of higher plants in nature are polyploid, and major grain, cotton and oil crops such as wheat, cotton, and rapeseed are also polyploid. increase, their output has doubled. [0004] But since Japanese scholar Nakamori discovered polyploid rice in 1933 (Nakamori, E. On the occurrence of the tetraploid plant of rice Oryza sativa L., Proc, Imp. Acad. 9, 340-341, 1933), there are many Scientists are engaged in research on rice polyploidy. Although polyploid rice has been found to have the advantages of thicker stems and larger grains, the breeding of polyploid rice has progressed slowly. Its main reason is that the seed-setting rate of polyploid rice is low, generally all is lower th...
Claims
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