Method of construction of allopolyploid rice through combination of embryo rescue and in-vitro induction

Abstract

The invention provides a method of construction of allopolyploid rice through combination of embryo rescue and in-vitro induction. The method includes the following steps: a, disposing wild rice under dark conditions to enable the flowering stage of the wild rice and the flowering stage of cultivated rice to converge; b, hybridizing the cultivated rice with the wild rice; c, after the hybridization, spraying young embryo development promoting fluid; d, carrying out embryo rescue cultivation; e, hybrid embryos send forth sprouts or calluses; f, treating the sprouts or the calluses with colchicines; i, transplanting tissue culture seedlings; j, selecting heterogenous hybrid seedlings; and k, determining allotetraploid rice or allohexaploid rice. Through the method, a plurality of species of heterogeneous hybrids and allopolyploids among a plurality of genomes can be obtained, and the method develops a new path for crossbreeding of cultivated rice / wild rice and full utilization of favorable genes of the wild rice.

Description

technical field [0001] The invention relates to a method for hybrid breeding of wild rice and cultivated rice, in particular to a method for breeding varieties of allopolyploid rice by combining embryo rescue and in vitro induction doubling after hybridization of wild rice and cultivated rice, belonging to The technical field of new crop variety selection and breeding in modern agriculture. Background technique [0002] Facing the challenge of the world food crisis, there is an urgent need for technologies and ideas for greatly increasing food production. However, for a long time, rice crops have mainly bred new varieties through hybridization between different varieties within the same species. Due to the long-term evolution and artificial selection of cultivated species, many good genes that originally existed in wild species have been eliminated, resulting in narrower and narrower genetic bases between hybrid parents and poorer genetic diversity (Meng Jinling. Plant Rep...

AI Technical Summary

Problems solved by technology

However, due to the limitation of the principle of sexual hybridization, it is difficult for the cultivated rice with genome A to hybridize with wild rice with 9 genomes, including BB, CC, BBCC, CCDD, EE, FF, GG, HHKK, HHJJ (Wang Aiyun, Chen Dongling, Cai Detian. Application of distant hybridization and allopolyploidization technology in rice breeding. Wuhan Botanical Research, 2005, 23(5): 491-495), favorable genes distributed in wild rice are difficult to be combined In cultivated rice with A genome

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