Method of construction of allopolyploid rice through combination of embryo rescue and in-vitro induction

An allopolyploid and embryo rescue technology, applied in the field of hybrid breeding of wild rice and cultivated rice, can solve problems such as difficulty in combining favorable genes

Active Publication Date: 2013-06-19
HUBEI UNIV +1
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  • Abstract
  • Description
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Problems solved by technology

However, due to the limitation of the principle of sexual hybridization, it is difficult for the cultivated rice with genome A to hybridize with wild rice with 9 genomes, including BB, CC, BBCC, CCDD, EE, FF, GG, HHKK, HHJJ (Wang Aiyun, Chen Dongling, Cai Detian. Application of distant hybridization and allopolyploidization technology in rice breeding. Wuhan Botanical Research, 2005, 23(5): 491-495), favorable genes distributed in wild rice are difficult to be combined In cultivated rice with A genome

Method used

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  • Method of construction of allopolyploid rice through combination of embryo rescue and in-vitro induction
  • Method of construction of allopolyploid rice through combination of embryo rescue and in-vitro induction
  • Method of construction of allopolyploid rice through combination of embryo rescue and in-vitro induction

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Embodiment 2

[0064] Embodiment 2. cultivated rice / tall wild rice ( Oryza alta ) Breeding of allohexaploid rice

[0065] a. Dark treatment of wild rice and the flowering period of cultivated rice

[0066] Tall wild rice ( Oryza alta ) is a typical short-day plant, under natural conditions in subtropical to temperate regions, it cannot be cultivated in rice ( Oryza sativa ) varieties bloom when they bloom. In order to make the flowering period of the two types of parents meet, the tall wild rice seedlings with more than 7 leaves were covered with a red and black double-layer cloth cover, and the light period was controlled at 8.5 hr / day. d, After a total of 30 days of treatment, O. sativa entered the reproductive growth period and flowered in early August, which coincided with the flowering period of cultivated rice varieties Sgdts96, HN2026 and Nipponbare.

[0067] b. Hybridization of cultivated rice and wild rice

[0068] Considering the characteristics of high-stalk wild rice fl...

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Abstract

The invention provides a method of construction of allopolyploid rice through combination of embryo rescue and in-vitro induction. The method includes the following steps: a, disposing wild rice under dark conditions to enable the flowering stage of the wild rice and the flowering stage of cultivated rice to converge; b, hybridizing the cultivated rice with the wild rice; c, after the hybridization, spraying young embryo development promoting fluid; d, carrying out embryo rescue cultivation; e, hybrid embryos send forth sprouts or calluses; f, treating the sprouts or the calluses with colchicines; i, transplanting tissue culture seedlings; j, selecting heterogenous hybrid seedlings; and k, determining allotetraploid rice or allohexaploid rice. Through the method, a plurality of species of heterogeneous hybrids and allopolyploids among a plurality of genomes can be obtained, and the method develops a new path for crossbreeding of cultivated rice/wild rice and full utilization of favorable genes of the wild rice.

Description

technical field [0001] The invention relates to a method for hybrid breeding of wild rice and cultivated rice, in particular to a method for breeding varieties of allopolyploid rice by combining embryo rescue and in vitro induction doubling after hybridization of wild rice and cultivated rice, belonging to The technical field of new crop variety selection and breeding in modern agriculture. Background technique [0002] Facing the challenge of the world food crisis, there is an urgent need for technologies and ideas for greatly increasing food production. However, for a long time, rice crops have mainly bred new varieties through hybridization between different varieties within the same species. Due to the long-term evolution and artificial selection of cultivated species, many good genes that originally existed in wild species have been eliminated, resulting in narrower and narrower genetic bases between hybrid parents and poorer genetic diversity (Meng Jinling. Plant Rep...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H4/00A01H1/04
Inventor 蔡得田王爱云杨春花杜超群张献华陈冬玲宋兆建王维何玉池刘育华
Owner HUBEI UNIV
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