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Method for identifying hypoxia tolerance and flooding tolerance of rice

An identification method and a technology of waterlogging tolerance, applied in the fields of botanical equipment and methods, germination equipment, application, etc., can solve the problems of lack of direct seeding rice varieties, inability to sow early, cold tolerance and low oxygen tolerance and waterlogging tolerance. , to achieve the significant effect of planting rice, saving cost and increasing efficiency

Inactive Publication Date: 2016-12-07
江西省农业科学院水稻研究所
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  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is a shortage of direct-seeding rice varieties, especially in double-cropping rice areas. There are basically no special direct-seeding varieties. The earing of double-cropping late rice is affected by the cold dew wind. Therefore, to solve the problem of direct-seeding rice varieties in double-cropping rice areas, it is necessary to enhance the germination characteristics of cold resistance and low oxygen resistance

Method used

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  • Method for identifying hypoxia tolerance and flooding tolerance of rice
  • Method for identifying hypoxia tolerance and flooding tolerance of rice
  • Method for identifying hypoxia tolerance and flooding tolerance of rice

Examples

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Embodiment 1

[0058] Screening and identification of rice varieties or breeding materials for tolerance to low oxygen and waterlogging, using "Ganxiang B / Dongxiang Wild Rice / / Ganxiang B" recombinant inbred line 47 stable lines, and selecting 30 plump seeds for each line or material , put them into water depth bottles (water depth 20-26cm), and then place them in a light incubator with a constant temperature of 25°C. Yu Ganxiang B was used as a control, from the germination of direct seeding dry seeds to the emergence of seedlings and growth on the water surface (December 15 to 12) 25th), the survival rate of the seedlings of the submerged varieties (lines) was 95%, and the non-submerged control could not grow leaves and stop growing after budding. like figure 1 , as shown in 2, is the situation diagram of germination and water production

Embodiment 2

[0060] The low-oxygen and flood-resistant varieties or strains were selected by screening, and crossed with excellent varieties. From the segregation generation population to the stable generation, each generation was identified for low-oxygen and water-logging resistance, and the low-oxygen and low-oxygen tolerance strains were selected as the selected strains. After 5-6 generations of selection and stabilization, new varieties (lines) resistant to hypoxia and flooding can be bred. like image 3 Identification of flood-tolerant germplasms of Ganxiang B and Dongxiang wild rice introduction lines shown

[0061] After 4 years of repeated experiments, 260 imported lines were tested for submergence tolerance. They were sown from dry seeds into deep water and treated for 20 days. The survival rate was 62.5-100%, and the control of Ganxiang B was 0.

Embodiment 3

[0063] Breeding of a new rice line 7676-1 resistant to hypoxia and flooding. This line is a hybrid and backcross progeny of the maintainer line Ganxiang B of "Yebai" as the female parent and Dongxiang wild rice as the male parent. In each generation, more individual plant materials were selected, and the selected materials were tested indoors. The above method was used to breed "7676-1", which is resistant to hypoxia and flooding. The 7676-1 strain was further tested and identified in a light incubator at a constant temperature of 15°C and 30°C. 40 plump dry seeds were selected and repeated three times. Compared with Ganxiang B. 15 days results, the germination rate is 100%, the seedling rate is 100%, the resistance to low oxygen and waterlogging is strong, and the results are consistent under the two temperatures. However, the emergence temperature is higher and the emergence is faster, otherwise it is slower, with a difference of 5-6%. like Figure 4 As shown, the mineral...

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Abstract

The invention provides a method for identifying hypoxia tolerance and flooding tolerance of rice. The method comprises the following steps: performing sexual hybridization and backcrossing on cultivated rice and Dongxiang wild rice with good hypoxia tolerance and flooding tolerance, selecting a signal plant material from separated descendants of the hybrid rice, putting full dried seeds into a water container with water of 20-26cm deep, putting the water container into an incubator of 25-30 DEG C, detecting water absorption, germination, budding and seedling emergency of seeds, enabling the rice growing out of the water level to grow normally, breeding for 5-6 generations, thereby breeding a novel variety of rice with good hypoxia tolerance and flooding tolerance. The method for identifying hypoxia tolerance and flooding tolerance of rice, which is provided by the invention, has the characteristics of being simple, convenient, accurate, easy to handle, and the like; the novel variety of rice identified by using the method is good in hypoxia tolerance and flooding tolerance, a novel direct seeding rice variety can be bred, and the method has great meanings of cost reduction, synergism and production and yield increase in grain production.

Description

technical field [0001] The invention relates to an identification method of crops, in particular to an identification method of rice tolerance to hypoxia and waterlogging. Background technique [0002] In my country's rice production, there are floods every year. There are severe and mild in the years, but they will bring different degrees of impact and loss on rice production. In severe cases, floods will destroy seedlings and fail to harvest, and in light cases, it will affect the growth and development of rice. Bring about a reduction in production and income. Especially in the double-cropping rice area in the middle and lower reaches of the Yangtze River in the south, it is the rainy season in the middle and late June of early rice every year. When floods cause disasters, the low-lying early rice fields are blocked by deep water or even submerged, and the middle rice seedlings are flooded. Often, when the submersion time exceeds a critical value, serious losses of dead s...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01C1/02
CPCA01H1/02A01C1/025A01H1/04
Inventor 陈大洲肖叶青罗世友陈红萍吴小燕胡兰香熊焕金邓伟陈明亮沈雨民
Owner 江西省农业科学院水稻研究所
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