QTL-seq based method for mining cold-tolerant gene of Dongxiang wild rice

Abstract

The invention discloses a QTL-seq based method for mining a cold-tolerant gene of Dongxiang wild rice. According to the method, cultivated rice 'Xieqing Zao B' is taken as a receptor parent, namely, a female parent; the Dongxiang wild rice is taken as a male parent; a recombinant inbred line BC1F10 obtained by distant hybridization and continuous backcrossing is subjected to cold tolerance identification; obtained 20 cold-tolerant strains form a cold-tolerant gene pool; and 20 cold-sensitive strains form a cold-sensitive gene pool. The two gene pools are subjected to high-throughput sequencing and QTL-seq analysis to obtain differential SNP makers and cold-tolerant character related QTL loci of the two gene pools; candidate cold-tolerant genes are subjected to fluorescent quantitative PCR detection; and finally the Dongxiang wild rice is detected to contain five cold-tolerant related genes by identification. According to the method, the cold-tolerant genes of the Dongxiang wild rice can be quickly and accurately detected and mined, a powerful evidence can be provided for comprehensively illuminating a genetic mechanism of cold tolerance character of the Dongxiang wild rice, a foundation is laid for cloning and functional analysis of the cold-tolerant genes of the Dongxiang wild rice, and the breeding practice of the cold-tolerant genes of the Dongxiang wild rice is facilitated.

Description

technical field [0001] The present invention relates to a method for discovering cold-tolerant genes of Dongxiang wild rice based on QTL-seq. Background technique [0002] Chilling injury is one of the main disasters in rice production, and it is an important limiting factor affecting the growth and development of rice in early spring and high latitude regions. When encountering cold weather below 10-15°C, mild cases will cause yellowing of rice seedlings and slow growth; severe cases will cause phenomena such as rotten seedlings, rotten buds and dead seedlings, which seriously affect the production of early rice. About 1 million hm per year worldwide 2 Rice suffers from cold damage and chilling damage in different growth periods. Therefore, the cold tolerance of rice has become a topic of common concern to breeders in the new era, and it is believed that the selection of cold-avoiding and cold-tolerant varieties is the most effective way to solve the chilling injury of ri...

AI Technical Summary

Problems solved by technology

[0005] Traditional QTL mapping methods rely on the construction of mapping populations and the development of molecular markers. This process takes a long time and involves a lot of work, which seriously restricts the efficiency and accuracy of QTL mapping.

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