Method for improving the seedling-becoming ratio hybridized with planting rice and different genome type wild rice

Abstract

The invention relates to a method, belonging to the plant cross breeding technology, which aims to solve the problem that affinity does not exist in the hybrids between the cultivated rice and the wild rice with various genome types; the invention is disclosed to improve the rate of immature embryos. The invention adopts the technical proposal that by temperature and humidity regulation during heading and flowering stage and the application of wind proofing facilities, a large amount of high-activity fresh pollen of the wild rice with various genome types is acquired; then deep color bagging or hot water treatment is utilized to adjust the flowering time encounter of male parent and female parent; the anther of singular floret is clipped by a nipper, or the spikelet is arranged on the female parent panicle to be pollinated, and then the spikelet is shaken by hand; the pollen utilization ratio of the wild rice with various genome types is improved, and the effect is better after two or three times of repeated pollination. By utilizing the hybridization between various types of cultivated rice material and wild rice with various genome types, materials with good affinity are selected to be hybridized with wild rice with various genome types. The invention has the advantages of simple operation, low cost, and the improvement of the rate of immature embryos from 0 to 40% to 30% to 60%; the method preferably solves the problem that affinity does not exist in the hybrids between the cultivated rice and the wild rice with various genome types, and the rate of immature embryos is greatly improved.

Description

Technical field: [0001] The patent of the present invention belongs to the plant hybrid breeding technology. It specifically relates to a new method that can significantly improve the hybridization rate of cultivated rice and wild rice with different genotypes. Background technique: [0002] Wild rice with different genome types covers nine genomes of B, C, D, E, F, G, H, J, and K, and is widely distributed all over the world. It has rich genetic diversity and many excellent traits. It is a natural rice gene The treasure house can provide abundant germplasm resources for broadening the genetic basis of rice. For example, the wild rice with karyotype CCDD (tetraploid) has the characteristics of resistance to rice blast, Philippine waterfly, borer and brown planthopper and other diseases and insect pests, and has high cold tolerance, shade tolerance and good growth in low light conditions It has great application value in rice breeding. However, so far, due to the distant g...

AI Technical Summary

Problems solved by technology

However, so far, due to the distant genetic relationship between wild rice of different genotypes and cultivated rice, the genetic differences are very large, there is a certain degree of reproductive isolation, the performance of wild crossing is not compatible, and the flowering habits of wild rice of different genotypes are different. Different from cultivated rice and ordinary wild rice, such as irregular flowering, only a few flowers per ear per day, and infrequent flowers, etc., it is difficult for technicians to obtain enough fresh pollen of wild rice with different genotypes when hybridizing in the field, which makes it different from cultivated rice. Rice or common wild rice hybridization has low embryonic rate, high difficulty and low efficiency