A synthetic method of wheat in winter wheat area with high genetic diversity and high embryo and seedling rate
A technology of genetic diversity and synthesis method, which is applied in the field of synthesis of wheat, winter wheat with high genetic diversity and high embryo and seedling rate, can solve the problem of limited genetic information, inapplicability of winter wheat area, and Low embryo seedling rate and other problems, to achieve the effect of broadening the genetic basis, wide selection range, and simple operation process
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Embodiment 1
[0033] A method for synthesizing wheat with high genetic diversity and high embryo and seedling rate, using the following steps:
[0034] A, select durum wheat and rough goat grass to sow by mass and number ratio 2:1, durum wheat is sowed in three phases, every 3 days at intervals, the first stage sowing of rough goat grass;
[0035] B, select durum wheat as the female parent, choose A. chinensis as the male parent, carry out emasculation and bagging to the durum wheat of the mother, and pollinate and bag the 48 hours after emasculation;
[0036] After c, pollination 24 hours, cut off 30cm below the ear of durum wheat plant, and after 30s with tap water flushing, insert in liquid culture medium I and cultivate, culture temperature is 21 ℃, and culture humidity is 80%, and light is 1500lux, 8 hours of light per day, after 14 days of cultivation, the wheat grains are stripped from the ears for sterilization;
[0037] d. Peel off the sterilized immature embryos of wheat grains o...
Embodiment 2
[0045] A method for synthesizing wheat with high genetic diversity and high embryo and seedling rate, using the following steps:
[0046] A, select durum wheat and rough goat grass to sow by mass and number ratio 2:1, durum wheat is sowed in three phases, every 5 days at intervals, the first stage sowing of rough goat grass;
[0047] B, select durum wheat as the female parent, choose A. chinensis as the male parent, carry out emasculation and bagging to the maternal durum wheat, 72 hours after emasculation, pollinate and bag with the newly collected A. chinensis pollen;
[0048] After c, pollination 24 hours, 40cm below the durum wheat plant ear is cut off, and after 30s with tap water flushing cut-off place, insert in liquid culture medium I and cultivate, culture temperature is 25 ℃, and culture humidity is 80%, and light is 2500lux, 8 hours of light per day, after 14 days of cultivation, the wheat grains are stripped from the ears for sterilization;
[0049] d. Peel off th...
Embodiment 3
[0057] A method for synthesizing wheat with high genetic diversity and high embryo and seedling rate, using the following steps:
[0058] A, select durum wheat and rough goat grass to sow by mass and number ratio 2:1, and durum wheat is sowed in three phases, every 4 days at intervals, the first stage sowing of rough goat grass;
[0059] B, select durum wheat as the female parent, choose A. chinensis as the male parent, carry out emasculation and bagging to the durum wheat of the mother, 51 hours after emasculation, pollinate and bag with the pollen of the newly collected A. chinensis pollen;
[0060] After c, pollination 24 hours, cut off 37cm below the ear of durum wheat plant, and after 30s with tap water flushing cut-off place, insert in liquid culture medium I and cultivate, culture temperature is 22 ℃, and culture humidity is 80%, and light is 1900lux, 8 hours of light per day, after 14 days of cultivation, the wheat grains are stripped from the ears for sterilization; ...
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