Disclosed are: a method for detecting
common wheat among from wheat varieties contained in a sample of interest such as a food
raw material or a processed food specifically, with high sensitivity, and in a qualitative and / or quantitative manner; a method for discriminating between
common wheat and a wheat variety other than
common wheat (e.g., durum wheat) contained in a food
raw material or a processed food and detecting the common wheat in a qualitative and / or quantitative manner; and a primer set, a
nucleic acid probe, and a detection kit, each of which can be used in the methods employing a
PCR method. Specifically disclosed are: a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a
PCR method using a
nucleic acid extracted from the sample as a template and using a primer comprising the
nucleotide sequence represented by SEQ ID NO:5 and a primer comprising the
nucleotide sequence represented by SEQ ID NO:6 and detecting the occurrence of a PCR amplification product; and a method for detecting the occurrence of common wheat in a sample of interest, which comprises carrying out a quantitative
PCR method using a
nucleic acid extracted from the sample as a template and using a primer comprising the
nucleotide sequence represented by SEQ ID NO:5, a primer comprising the nucleotide sequence represented by SEQ ID NO:6 and a nucleic acid probe comprising the nucleotide sequence represented by SEQ ID NO:11 and detecting the occurrence of common wheat qualitatively and / or quantitatively.