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Creation and in-situ hybridization of wheat-elymus hispidus multi-disomic substitution line containing elymus hispidus 1Ns, 5Ns and 6Ns chromosomes, simple sequence repeat (SSR) and polyacrylamide gel electrophoresis (PAGE)

A technology of heterosubstitution line and in situ hybridization, which is applied in the preparation of test samples, the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problem of undetermined origin of Xm genome

Inactive Publication Date: 2014-02-19
NORTHWEST A & F UNIV
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Problems solved by technology

The genome of Limegrass is NsNsXmXm, where the Ns genome comes from Psathyrostachys huashanica Keng, and the source of the Xm genome has not yet been determined

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  • Creation and in-situ hybridization of wheat-elymus hispidus multi-disomic substitution line containing elymus hispidus 1Ns, 5Ns and 6Ns chromosomes, simple sequence repeat (SSR) and polyacrylamide gel electrophoresis (PAGE)
  • Creation and in-situ hybridization of wheat-elymus hispidus multi-disomic substitution line containing elymus hispidus 1Ns, 5Ns and 6Ns chromosomes, simple sequence repeat (SSR) and polyacrylamide gel electrophoresis (PAGE)
  • Creation and in-situ hybridization of wheat-elymus hispidus multi-disomic substitution line containing elymus hispidus 1Ns, 5Ns and 6Ns chromosomes, simple sequence repeat (SSR) and polyacrylamide gel electrophoresis (PAGE)

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Embodiment Construction

[0022] The preferred embodiments of the present invention will be described in detail below; it should be understood that the preferred embodiments are only for illustrating the present invention, rather than limiting the protection scope of the present invention.

[0023]Through embryo rescue and colchicine chromosome doubling, the common wheat-Lime wheat incomplete amphidiploid M842 with the characteristics of large spikes, multiple flowers, large grains, and disease resistance of Lime wheat was obtained; Wheat hybridization, 3 heteroaddition lines and 2 heterosubstitution lines were bred; genome in situ hybridization was used to conduct molecular cytogenetic analysis on the genome composition of 6 types of octoploid Tritileymus , the genome composition of octoploid wheat M842-4, M842-8, M842-12 and M842-16 was determined as AABBDDNsNs, the genome composition of M842-10 was determined as AABBDDNsNs+2Ta-2Lm(Ns), M842 The genome composition of -13 was determined to be AABBDDJJ...

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Abstract

The invention discloses creation of a wheat-elymus hispidus multi-disomic substitution line containing elymus hispidus 1Ns, 5Ns and 6Ns chromosomes. Octoploid tritileymus M842-12 (2n=8x=56, AABBDDNsNs) is utilized to hybridize together with durum wheat (2n=4x=28, AABB) variety Trs-372; cytology chromosome microscopy and genomic in situ hybridization (GISH) identification are carried out on F1, F2, F3 and F4 generations, so as to screen a single plant, of which the quantity of the chromosomes accords with the formula: 2n=42, and the single plant contains an elymus hispidus Ns gene genome chromosome; the screened single strain is bagged to inbreed, so that the wheat-elymus hispidus multi-disomic substitution line 05DM6 containing the elymus hispidus 1Ns, 5Ns and 6Ns chromosomes is bred in the F5 generation.

Description

technical field [0001] The present invention relates to creation, in situ hybridization, SSR and PAGE identification of a wheat-bentia multiple heterosubstitution line comprising 1Ns, 5Ns and 6Ns chromosomes. Background technique [0002] Wheat distant hybridization is one of the important ways and means to use exogenous genetic material to enrich the genetic basis of wheat and artificially create new species. Lime wheat (Leymus mollis (Trin.) Hara), also known as soft Lymus, belongs to Poaceae, Barley subfamily, Lymus is a wild weed, with drought resistance, cold resistance, salt resistance, barren resistance, resistance to a variety of fungi, Bacterial diseases, strong stalks, large spikes and multiple flowers are excellent germplasm resources for wheat crop variety improvement [2，3] . The genome of Limegrass is NsNsXmXm, and the Ns genome comes from Psathyrostachys huashanica Keng, while the source of the Xm genome has not yet been determined. [0003] In the 1960s, Ts...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/30G01N27/447C12Q1/68
Inventor 赵继新陈新宏庞玉辉杜万里武军程雪妮杨群慧
Owner NORTHWEST A & F UNIV
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