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Breeding method of high-hardness wheat

A high-hardness, wheat-based technology, applied in the fields of crop genetics and breeding and agricultural biology, can solve the problems of difficult identification of large and small cytology, and achieve the effects of stable genetic transmission, increased hardness, increased protein content and lysine content

Active Publication Date: 2021-01-29
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

This application further determined the translocation breakpoint, and located the translocation breakpoint on 2RS at FL 0.6 of 2RS, and the breakpoint on 5D was located at the AFA family signal region at the top of 5DS, but the specific size cytology is difficult to identify

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  • Breeding method of high-hardness wheat
  • Breeding method of high-hardness wheat
  • Breeding method of high-hardness wheat

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Embodiment Construction

[0047] 1. Accurate identification of translocation breakpoints of wheat-Jingzhou rye translocation line T5DL·5DS-2RS

[0048] Using rye genome in situ hybridization, oligonucleotide probe set #4 (see references: Du P, Zhuang L, Wang Y, Yuan L, Wang Q, Wang D, Dawadondup, Tan L, Shen J, Xu H, Zhao H, Chu C, QiZ. Development of oligonucleotides and multiplex probes for quick and accurate identification of wheat and Thinopyrum bessarabicum chromosomes[J]. Genome, 2017, 60:1-11.) and oligonucleotide probe set #5 ( See Reference: Lei Yanhong. Identification and Application of Oligonucleotide Probe Sets for Simultaneously Identifying Chromosomes of Wheat and Rye or Wheat and Trichophyllum [D]. Master's Thesis of Nanjing Agricultural University, 2019.) to Yi FISH / GISH analysis of mitotic metaphase chromosomes in root tip cells of T5DL·5DS-2RS and its F2 hybrid seeds ( figure 1 ), two translocation chromosomes were found, which were homozygous translocation chromosomes. The transloca...

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Abstract

The invention discloses a breeding method for high-hardness wheat. The wheat bred according to the method contains a wheat-rye translocation chromosome T5DL.5DS-2RS, and the translocation chromosome lacks hardness genes Pina and Pinb carried by 5DS and a soft protein gene Gsp1, so that the grain hardness is remarkably improved, the protein content is high, and the method is a new method for breeding common wheat similar to the hard-grained wheat. One disclosed molecular marker combination can accurately distinguish T5DL-5DS-2RS homozygotes, heterozygotes and non-translocation single plants atthe same time through one-time amplification, marker-assisted selection and utilization of the T5DL.5DS-2RS in high-hardness wheat breeding are facilitated, the new method is provided for wheat quality improvement, and the breeding method belongs to the technical field of crop genetic breeding and agricultural biology.

Description

[0001] 1. Technical field [0002] The patent discloses a breeding method for high-hardness wheat, which belongs to the field of crop genetic breeding and agricultural biotechnology. The application found that the wheat-rye translocation line T5DL·5DS-2RS lacks the hardness genes Pina, Pinb and soft protein gene Gsp-1 carried by 5DS at the same time, so the grain hardness (hardness index 83.28±1.63) It was significantly higher than Yangmai 158 (68.32±2.45) and Yannong 19 (71.25±0.82), and the grain protein content was increased. Two hybrids F 3 In the T5DL·5DS-2RS homozygous subpopulation, the hardness index of the subpopulation without this translocation was significantly increased by 11.70 and 16.34, respectively, reaching 79.60±7.27 and 85.27±4.11. At the same time, it can accurately distinguish T5DL·5DS-2RS homozygous, heterozygous and non-translocation individual plants, which is conducive to the marker-assisted selection and utilization of T5DL·5DS-2RS in high hardness wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 亓增军刘鑫张慧婷
Owner NANJING AGRICULTURAL UNIVERSITY
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