Wild-rice distant hybridization high-efficient cultivating superior progeny method

A technology of distant hybridization and wild rice, applied in the field of plant cultivation, can solve the problems of difficulty in character stability, difficulty in hybridization between cultivated rice and wild rice, and difficulty in receiving hybrid seeds, so as to speed up the homozygous stability of traits and shorten the breeding period. , accelerate the effect of homozygous

Inactive Publication Date: 2008-03-19
云南省农业科学院生物技术与种质资源研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The karyotype of common wild rice is A'A'; the karyotype of wild rice is CC; the karyotype of wild rice is GG, while the karyotype of cultivated rice is AA. It

Method used

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  • Wild-rice distant hybridization high-efficient cultivating superior progeny method
  • Wild-rice distant hybridization high-efficient cultivating superior progeny method
  • Wild-rice distant hybridization high-efficient cultivating superior progeny method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] 1. Hybridization, embryo rescue and strong seedling cultivation of cultivated rice

[0068] 1) The cultivated rice is sown in batches to adjust the flowering period of the cultivated rice to meet that of the wild rice. In the wild hybridization, cultivated rice was used as the female parent, and Yuanjiang common wild rice was used as the male parent for hybridization. Before the cultivated rice blooms, usually in the morning, cut off 1 / 3 of the chaff with scissors, and then use tweezers to pick out the anthers and then bag them to prevent powder clustering. Before the female parent is pollinated, a certain amount of female parent pollen is collected to make it die naturally (the rice pollen will die in a short time after it is emitted from the anther). The dead cultivated rice pollen is mixed with wild rice pollen to pollinate and bag the female parent. In this method, the recognition protein on the pollen of cultivated rice can still induce the stigma to accept the p...

Embodiment 2

[0115] The method is basically the same as the above-mentioned embodiment 1, the difference is:

[0116] 1. The parental material of wild rice is another important type of wild rice in Yunnan, namely Jinghong wild rice.

[0117] One, 1) in: from the 2nd day of pollination, spray the GA3 (gibberellin) solution of 100mg / L to the pollination ear continuously for 3-5 days, spray 3 times every day.

[0118] 1. 2) During rice embryo rescue: the composition of medium WJ2 is: MS+NAA0.5mg / L+KT1mg / L+30g / L sucrose+9g / L agar, pH5.8.

[0119] 2. The pollen cultivation of wild hybrid offspring:

[0120] 4) Middle: The pollen callus induction medium IN formula was adjusted to: N6+2,4-D 3.0 mg / L+KT 1.0 mg / L+sucrose 30 g / L+agar 9 g / L, pH 5.8.

[0121] 5) Medium: The composition of the subculture medium SUB is: N6+2,4-D2.5mg / L+KT1mg / L+sucrose 30g / L+agar 9g / L, pH5.8;

[0122] 6) Medium: The callus after subculture for 2 weeks was treated with 0.5% colchicine (sterilized by filtration) under s...

Embodiment 3

[0127] The method is basically the same as the above-mentioned embodiment 1, the difference is:

[0128] 1. 2) During rice embryo rescue: the composition of medium WJ2 is: MS+NAA0.25mg / L+KT2mg / L+30g / L sucrose+9g / L agar, pH5.8.

[0129] 2. The pollen cultivation of wild hybrid offspring:

[0130] 4) Middle: The pollen callus induction medium IN formula was adjusted to: N6+2,4-D2.5mg / L+KT0.5mg / L+sucrose 30g / L+agar 9g / L, pH5.8.

[0131] 5) Medium: The composition of the subculture medium SUB is: N6+2,4-D2.0mg / L+KT0.6mg / L+sucrose 30g / L+agar 9g / L, pH5.8;

[0132] 6) Medium: The callus after subculture for 2 weeks was treated with 0.3% colchicine (sterilized by filtration) under sterile conditions, and then put back into the incubator at 26°C for 72 hours, and the excess was sucked off after completion. colchicine, inoculated on differentiation medium to regenerate plants.

[0133] 7) Middle: The formula of DH plant differentiation medium RE is adjusted to: N6+KT2.0mg / L+BA0.5mg / L...

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Abstract

The invention relates to a method for high-efficiently cultivating excellent later generation of the wild rice distant hybridization , which is to cultivate the wide rice distant-hybridization high-efficient excellent later generation through the hybridization of the cultivated rice, the embryo rescue, the strong seedling culture, the pollen culture and the molecular marker assisted selection of the hybridization later generation of the cultivated rice and the wide rice. The survival rate of the distant hybridization of the invention is high; the hybrid progeny is backcrossed at the preliminary period and then self-bred, thus being beneficial to overcome the reproductive barrier caused by the different genome in the hybrid progeny of the wide rice and the cultivated rice, and to stabilize more genetic characters of the wide rice inside the cultivated rice; the pollen culture can accelerate the stability of the purification of the hybrid progeny; with the established molecular marker assisted selection, the breeding can be accurately performed without being influenced by the environmental conditions and being restricted by the growth and development period.

Description

technical field [0001] The invention relates to plant cultivation technology, in particular to a method for efficiently cultivating excellent offspring by distant hybridization of wild rice. Background technique [0002] Rice distant hybridization is the hybridization between rice families with different genome types in terms of botanical morphological classification, called rice distant hybridization. Distant hybridization breaks down the boundaries between species to a certain extent. Rice belongs to the genus Oryzae of the family Poaceae, and 23 species have been found. There are 3 kinds of wild rice distributed in China, and there are 3 kinds of wild rice in China in Yunnan, namely common wild rice, medicinal wild rice and verrucous wild rice. [0003] Distant hybridization of rice is the use of wild rice and cultivated rice to exchange genes artificially, so as to integrate different excellent and unique traits into a hybrid individual to varying degrees to create new...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/04A01H1/08A01H4/00C12N5/04C12Q1/68
Inventor 程在全黄兴奇殷富有孙一丁丁玉梅
Owner 云南省农业科学院生物技术与种质资源研究所
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