Method for protection and breeding of medicinal wild rice
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A technique of medicinal wild rice and a culture method, which is applied in the field of plant tissue culture and can solve the problems of callus, differentiated tissue culture seedlings and the like
Active Publication Date: 2018-02-09
云南省农业科学院生物技术与种质资源研究所
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Problems solved by technology
[0006] The purpose of the present invention is to overcome the thorny problem that the mature seeds of medicinal wild rice are difficult to induce callus and differentiate into tissue culture seedlings, and solve and provide a cultivation method for the rapid propagation of medicinal wild rice mature seeds from practice
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Embodiment 1
[0027] Example 1: Rapid Propagation and Cultivation of Mature Seeds of Wild Rice for Medicinal Use in Jinghong, Yunnan
[0028] In Example 1, the mature seeds of the medicinal wild rice in Jinghong, Yunnan were used as experimental materials, and the regenerated plants of the medicinal wild rice were obtained by using this method. The specific method of implementation is as follows:
[0029] 1) Selection of explants: the mature seeds harvested in the same year were selected as explants, packed in paper bags, and stored in a cool and dry place for later use;
[0030]2) Seed treatment: Take out the spare seeds packed in paper bags, peel off the chaff, and leave the complete rice grains, use a small triangular file to make a shallow concave on the surface of the rice grains, and put the concave rice grains into a sterile 50mL In the triangular flask, set aside;
[0031] 3) Seed sterilization treatment: place a 50mL triangular flask with concave rice grains on a sterile table, w...
Embodiment 2
[0046] Example 2: Rapid Propagation and Cultivation of Mature Seeds of Wild Rice in Lincang, Yunnan
[0047] In Example 2, the mature seeds of Oyza officinalis in Lincang, Yunnan were used as experimental materials, and regenerated plants of Oyza officinalis were obtained by this method. This embodiment is basically the same as Embodiment 1, the difference is:
[0048] 1) Seed treatment: Take out the spare seeds packed in paper bags, peel off the chaff, and leave the complete rice grains. The rice grains are ground with fine sandpaper, washed 4 times with sterile water, dried with filter paper, and put Put it into a triangular flask and set aside;
[0049] 2) Callus induction culture: After aseptically treating the seeds to be cleaned with epidermis, directly insert them on the callus induction medium, and place them in an artificial climate box with a temperature of 28°C for dark culture of callus induction. The formula of the induction medium is: MS basic medium + 2,4-D 5m...
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Abstract
The invention discloses a method for protection and breeding of medicinal wild rice and belongs to the technical field of plant tissue culture. The method for protection and breeding of medicinal wildrice comprises steps as follows: explant selection, seed treatment, seed sterilization, seed germination acceleration, seed bud callus induction, callus material treatment, callus induction culture,callus subculture, differentiation culture, primary subculture differentiation culture, secondary subculture differentiation culture, differentiation multiplication culture, strong seedling culture, rooting culture, seedling hardening culture, greenhouse transplantation of seedlings, field transplantation of the seedlings and field seedling management. The problem of difficulty in seed callus induction and seedling differentiation of the wild rice is solved, the callus rate and the differentiation rate are higher through induction, and medicinal wild rice seedlings obtained through tissue culture and rapid propagation grow well. The method provides powerful technical support for seedling differentiation of the medicinal wild rice and other rice varieties with difficulty in tissue culture through tissue culture and rapid propagation of seeds and also provides an effective method for protection and utilization of the medicinal wild rice.
Description
Technical field: [0001] The invention relates to a method for protecting and breeding medicinal wild rice, which belongs to the technical field of plant tissue culture. Background technique: [0002] Wild rice is the original ancestor of cultivated rice. It has grown in the wild for a long time and has withstood the selection of various adversity stresses. It has rich genetic diversity and preserves a large number of excellent genes that cultivated rice does not have or has disappeared. It has important academic value and economic value. value. [0003] There are common wild rice, medicinal wild rice and verrucous wild rice in China. Among them, medicinal wild rice (Oryza officinalis Wall) is the resource with the most research value. It has the strongest growth potential among the 22 species of Oryza genus, which is more than 20 times that of ordinary cultivated rice. It has super large panicles, super wide leaves, and super thick stems. Its genome and genotype are very d...
Claims
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