Specific Identification Method of Watermelon Variety "Jingmei"

Abstract

The invention discloses a specificity identification method for a watermelon variety 'Jingmei'. The specificity identification method for the watermelon variety 'Jingmei' comprises steps as follows: with genome DNA of the watermelon variety 'Jingmei' as a template, PCR amplification is performed by the aid of 28 core SSR primer pairs, amplified products are subjected to polyacrylamide gel electrophoresis, and a '0,1' matrix is constructed according to an electrophoretogram (in positions where the gel migration rates are the same, positions with bands are marked as 1 and positions without bands are marked as 0); the '0,1' matrix of a to-be-tested watermelon is obtained with the same method; the '0,1' matrix is of the to-be-tested watermelon and the '0,1' matrix of 'Jingmei' are compared. The constructed '0,1' matrix of 'Jingmei' is totally different from that of other varieties, can be used for sensitively distinguishing the variety 'Jingmei' from other varieties at high resolution and can be used for identifying authenticity of the variety 'Jingmei', and an effective scientific basis is provided for intellectual property protection of the variety 'Jingmei'.

Description

technical field [0001] The invention belongs to vegetable breeding and application technology, and relates to a specific identification method for a watermelon variety "Jingmei". Background technique [0002] Watermelon is not only an efficient horticultural crop for farmers in Beijing to increase their employment and income, but also an important seasonal fruit to meet the needs of urban and rural residents. With the vigorous development of the watermelon industry, the demand for high-quality watermelon varieties is increasing. However, driven by huge interests, the watermelon market is chaotic, and the phenomenon of "same thing with different name, same name with different thing" is common, which brings great difficulties to the protection of intellectual property rights of watermelon varieties, seed quality management and identification of new varieties. [0003] In order to regulate the market and protect the rights and interests of breeders, scientific identification a...

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Problems solved by technology

[0004] Traditional variety-specific testing is mainly based on morphological characteristics, which is greatly affected by the environment, has poor stability, and has a long test cycle, which seriously affects the effectiveness and authority of specificity testing.

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