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A kind of identification method of snake bile

A technology for snake bile and snake bile is applied in the identification field of snake bile, which can solve the problems of thin bile and poor quality, and achieve the effects of high accuracy, control of drug quality, and quality control.

Active Publication Date: 2021-09-03
广西壮族自治区食品药品检验所
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Counterfeit products of snake gallbladder are found from time to time in the market. The adulterated substances reported include bile and starch paste of chicken, duck, fish, pig, cattle and other animals, vegetable juice, gelatin solution, pigment, rehmannia glutinosa extract, etc., and oil gall ( Stimulate the snake's nasal cavity or body with engine oil to increase the bile quality) or honey gallbladder or soak the snake gallbladder for many times, then sell the snake gallbladder and wine separately to make the bile thin and poor in quality

Method used

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  • A kind of identification method of snake bile
  • A kind of identification method of snake bile
  • A kind of identification method of snake bile

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Embodiment 1

[0022] A method for identifying snake bile, the method comprising the following:

[0023] 1. Instruments and reagents:

[0024] (1) Snake bile material: Snake samples were collected from 20 species of live snakes in Guangdong, Guangxi, etc., slaughtered to obtain snake gall, and stored in ethanol with a volume concentration of 50% at a weight ratio of 1:1. The other 7 species of non-snake animals The gallbladder was purchased from the vegetable market in Nanning, Guangxi. Snake samples were identified by Huang Qingquan, the chief pharmacist of Guangxi Food and Drug Inspection Institute, and the certificate specimens were kept in the specimen room of Guangxi Food and Drug Inspection Institute, see Table 1

[0025] Table 1 Test materials

[0026] Table 1 Source of materials

[0027]

[0028] (2) Instruments: S1000 PCR amplification instrument (Applied Biosystem Company); Eppendorf Mastercycler® nexus gradient; electrophoresis instrument (BIO-RAD); trace ultraviolet spectro...

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Abstract

The invention discloses a method for identifying snake bile. The method finds out the specific DNA fragment of snake bile, designs specific primers, and amplifies the specific cytb fragment of snake bile through PCR technology, so as to identify other The bile of animals can be distinguished, and the authenticity of snake bile can be quickly and effectively identified. The identification method of the invention is simple to operate, has good repeatability and high accuracy, can effectively control the quality of snake bile, and provides scientific basis for rational use of snake bile.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a method for identifying snake bile. Background technique [0002] Snake gallbladder is first recorded in "Famous Doctors", which are anaconda gallbladder and pit viper gallbladder. "Chinese Pharmacopoeia" 20L5 Edition Four Volumes records that snake bile is the bile of various snakes in the family Cobra, Viperidae or Viperidae, but the current medicinal source of snake gall is far beyond the above range. Due to the unique medicinal value of snake gallbladder, coupled with the large market demand and diminishing natural resources, there are many traditional Chinese medicine preparations containing snake gallbladder on the market. Bei loquat cream, etc. Counterfeit products of snake gallbladder are found from time to time in the market. The adulterated substances reported include bile and starch paste of chicken, duck, fish, pig, cattle and other animals, ve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6806C12Q1/686
CPCC12Q1/6806C12Q1/686C12Q2521/537C12Q2527/125C12Q2565/125
Inventor 张涛罗轶吴桂凡黄莉莉李立陆游朱雪妍
Owner 广西壮族自治区食品药品检验所
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