The Method of Identifying Huaimai 33 Wheat Variety

Abstract

The invention belongs to the technical field of variety identification of wheat germplasm resources, and particularly relates to a method for identifying Huai wheat variety 33. By an SSR labeling technology, through screening of 800 pairs of SSR primers distributed on 21 chromosomes of wheat, barc45 and wmc382 primers are determined to have relatively high specificity to the Huai wheat variety 33 and are used for identifying the Huai wheat variety 33. By the method, the number of required detection samples is small; the method is mature and high in accuracy; and when the method is applied to identification of the Huai wheat variety 33 in the market, new variety promotion and germplasm resource protection are facilitated.

Description

technical field [0001] The invention belongs to the technical field of wheat germplasm resource variety identification, and in particular relates to a method for identifying Huaimai 33 wheat varieties. Background technique [0002] Wheat (Triticum aestivum L.) is one of the most important food crops in my country, with an annual planting area of ​​about 360 million mu. With the improvement of the commercialization level of seeds and the continuous emergence of new fine varieties, the replacement of wheat varieties in my country is getting faster and faster. Moreover, with the acceleration of rural land circulation in my country, the rate of farmers purchasing improved varieties is also increasing, and the annual demand for improved wheat varieties is about 5 billion catties. The existence of commercial value has led many seed companies to start operating improved varieties of wheat. In order to obtain high profits, companies without variety rights often engage in illegal ac...

AI Technical Summary

Problems solved by technology

[0003] The traditional morphological identification of wheat grains, seedlings, and plants requires experienced experts, and there are often problems such as long identification time and being easily affected by human subjective factors, so the court should not use it; phenol staining method, sodium hydroxide measurement, etc. Chemical identification methods are easily restricted by the degree of seed development and environmental conditions, and the identification accuracy is not high; protein labeling techniques such as acidic polyacrylamide gel electrophoresis often have many bands, the results are not easy to judge, and it is difficult to determine the close relationship. identification of varieties; the whole genome sequencing of a single variety often has many problems such as technical complexity and high cost

The shortcomings of the above techniques make it difficult to use them as a unified identification method for different wheat varieties

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