Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for building Slc26a4 gene L236P mutation mouse model

A slc26a4 and mouse model technology, applied in genetic engineering, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems such as phenotypes that cannot simulate hearing and balance

Inactive Publication Date: 2020-06-23
SHANDONG POLYTECHNIC
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But these phenotypes do not mimic the more subtle hearing and balance phenotypes shown in human patients

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for building Slc26a4 gene L236P mutation mouse model
  • Method for building Slc26a4 gene L236P mutation mouse model
  • Method for building Slc26a4 gene L236P mutation mouse model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0158] The following clearly and completely describes the technical solutions in the embodiments of the present invention. Apparently, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0159] In the embodiment of the present invention, a Slc26a4 The method for establishing a gene L236P mutant mouse model is characterized in that the specific steps are as follows:

[0160] The first step, the construction of the plasmid

[0161] (1) Construction of Slc26a4-pX330 vector

[0162] 1) Target design

[0163] First find the sequence of the Slc26a4 gene, find the gene sequence on the sixth exon, find the three bases encoded by the 236th amino acid, and design a target according to this position, according to the upstream ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for building an Slc26a4 gene L236P mutation mouse model. The method comprises the steps of firstly, finding a target sequence determined on a sixth exon of an Slc26a4gene, transferring connected px330 plasmids and a repair template synthesized in vitro into nuclei of fertilized eggs, transplanting the fertilized eggs into oviducts of pseudopregnant female mice andwaiting for development to produce F0 pups; cutting off toes of the mice and carrying out genome extraction and sequencing; carrying out T / A cloning experiments on the mice containing mutations neara target and carrying out sequencing; mating male mice containing an L236P gene mutation with wild CBA / CaJ female mice to produce F1 heterozygous mice; and inbreeding the F1 heterozygous mice to produce offspring and carrying out PCR and sequencing on the offspring, thereby completing the building of the Slc26a4 gene L236P mutation mouse model.

Description

technical field [0001] The invention relates to the field of gene mutation animal model construction, specifically a Slc26a4 The establishment method of gene L236P mutant mouse model. Background technique [0002] There are many varieties and strains of mice, and they are the animals with the most strains among experimental animals. There are more than 250 inbred strains of mice commonly used in the world, all with different characteristics. There are more than 350 mutant strains of mice. In terms of color, there are white, gray, brown, yellow, black and so on. For example, wild coat color mice have C3H, CBA / N, etc.; black mice have C57BL / 6, C57BL / 10, C58 strains, etc.; gray mice have C57L, DBA / 2 strains, etc.; white mice have A, AKR, BALB / c, RF, SWR and other strains. [0003] There are also many strains that need to be bred to study various human diseases, such as mice for cardiovascular disease: DBA and other strains; autoimmune disease mice: NZB / N, NZB×NZW and othe...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/66A01K67/027
CPCA01K67/0275A01K2217/072A01K2227/105A01K2267/03C07K14/47C12N15/66C12N15/8509
Inventor 李珍祖高建刚
Owner SHANDONG POLYTECHNIC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com