Use of rice OsEBS gene for improving rice yield trait

A rice and genetic technology, applied in the field of transgenics, can solve the problems of BIP only involving

Inactive Publication Date: 2014-02-12
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, the biological functions of BIP are almost only related to its function in protein folding and matura...

Method used

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  • Use of rice OsEBS gene for improving rice yield trait
  • Use of rice OsEBS gene for improving rice yield trait
  • Use of rice OsEBS gene for improving rice yield trait

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Experimental program
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Effect test

Embodiment 1

[0054] 1. Select the OsEBS gene segment and the upstream 1.1kb regulatory segment (see SEQ ID NO.1 for its full-length genome sequence, and SEQ ID NO.2 for its coding sequence), and use PCR to amplify it from the introgression line BIL112, And synthesize the insert linker and clone it into the plant expression vector pCAMBIA1304 to obtain the OsEBS expression vector pCAMBIA1304 / OsEBS;

[0055] 2. Culture medium for inducing rice callus

[0056] (1) Induction and subculture medium: MS+2mg / L 2,4-D;

[0057] (2) Hyperosmotic medium: MS+2mg / L 2,4-D+46.67g / L sorbitol+46.67gL mannitol;

[0058] (3) The first round of screening medium: MS+2mg / L 2,4-D+30mg / L hygromycin;

[0059] (4) The second round of screening medium: MS+2mg / L 2,4-D+50mg / L hygromycin;

[0060] (5) Differentiation medium: MS+3mg / L 6-BA+0.5mg / L NAA+50mgL hygromycin;

[0061] (6) Rooting and strong seedling medium: 1 / 2MS+0.1mg / L NAA;

[0062] In the present invention, the medium contains 30gL sucrose+2.5gL agar, p...

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Abstract

The invention belongs to the field of transgenic technology, and relates to use of a rice OsEBS gene for improving rice yield trait. The rice yield OsEBS gene and homologous gene thereof are transformed into indica type rice genome to improve the yield trait of the rice, and the indica type rice Guichao2 is used as a transgenic receptor to construct an OsEBS transgenic plant; furthermore, pCAMBIA1304 is used as an expression carrier, and OsEBS gene regulatory region of Dongxiang wild rice and coding region thereof are inserted into downstream of polycloning sites of the pCAMBIA1304 carrier to obtain an OsEBS gene expression carrier. The test result shows that the plant height, secondary branch number, secondary branch seed number, stem seed number, single plant seed number, average seed number, single plant seed weight, biological yield, thousand seed weight, and seed density of the obtained rice OsEBS transgenic plant are obviously improved and perfected.

Description

technical field [0001] The invention belongs to the field of transgenic technology, and in particular relates to the use of rice OsEBS gene in improving rice yield traits. Background technique [0002] Rice is one of the most important food crops in the world, and about 2 / 3 of the world's population uses rice as a staple food. In the case of gradually decreasing arable land, in order to meet the growing population's demand for food, it is of great significance to increase the unit yield of rice. Rice yield traits are mainly composed of effective panicle number, grain number per panicle and thousand-grain weight; the above yield traits are quantitative traits (QTL), controlled by many genes with different effects. The methods for isolating and cloning the above-mentioned yield trait genes disclosed in the prior art generally use positional cloning technology, and then use constitutive expression vectors or tissue-specific expression vectors to introduce the target gene into ...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00
Inventor 罗小金董贤欣杨金水
Owner FUDAN UNIV
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