Process for preparing feruoylated oligosaccharide by enzymolysis of wheat bran

A technology of feruloyl oligosaccharides and wheat bran, which is applied in the field of enzymatic hydrolysis of wheat bran to prepare feruloyl oligosaccharides, which can solve the problems of no reports

Inactive Publication Date: 2006-10-04
JIANGNAN UNIV
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Problems solved by technology

Feruloyl oligosaccharides not only contain feruloyl groups, but also contain hydrophilic xylooligosomes. Wheth

Method used

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Embodiment Construction

[0030] Wheat bran was treated with high-pressure steam at 121 °C for 45 min to inactivate endogenous enzymes. Suspend the treated wheat bran in a certain volume of water, stir continuously at 60°C for 16h to make it fully swell, then add 75mL / kg bran heat-resistant α-amylase, and stir the mixture in a boiling water bath for 40min. After cooling the suspension to 60°C, adjust the pH to 7.5, then add 30mL / kg bran hydrolyzing protease Alcalase, stir continuously at 60°C for 30min, adjust the pH to 4.5, then add 35mL / kg bran refined glucoamylase, 60 Stir continuously at ℃ for 30min, centrifuge the mixture, discard the supernatant, and obtain a precipitate, which is a common method for preparing wheat bran insoluble dietary fiber by destarching and removing protein. The present invention specially adopts repeated washing of the precipitate with hot distilled water, Until the suspension is not turbid when washed with cold distilled water, it is washed twice with hot distilled water,...

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Abstract

The invention relates to a method for enzymolyzing wheat bran to prepare asafetida oligosaccharide, wherein it uses wheat bran as raw material; uses enzymatical to remove amidon, remove protein to prepare wheat bran insoluble fiber; then uses bacillus subtilis xylanase to hydrolyze wheat bran insoluble fiber to prepare the asafetida oligosaccharide. And the density of asafetida oligosaccharide will reach 1.497mmol/L. the invention can effectively utilize wheat bran, while prepared asafetida oligosaccharide has significant biological activity, to accelerate the generation of bifidobacteria, and restrain the erythrocyte oxidisability damage indused by free group, with better social and economic benefits.

Description

technical field [0001] The invention discloses a method for preparing feruloyl oligosaccharides by enzymatically hydrolyzing wheat bran, which belongs to the technical field of resource development of agricultural by-products, functional food additives and nutritional health products. Background technique [0002] Wheat is one of the most important food crops in the world, especially in developed countries, and is an important part of human diet. my country's wheat production ranks among the top in the world. In recent years, my country's annual processing of wheat bran is about 20 million tons. Wheat bran is a good source of human dietary fiber, but its economic value as a feed component has declined significantly. There have been many foreign literatures reporting that wheat bran is beneficial to human body health as human dietary fiber. In recent years, research on wheat bran has focused on the physiologically active substances of wheat bran, such as arabinoxylan, phyti...

Claims

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Application Information

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IPC IPC(8): C12P19/00C12R1/07
Inventor 姚惠源袁小平陈正行马晓军
Owner JIANGNAN UNIV
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