Camellia sinensis(L.)O.Kuntze) WRKY29 gene and application thereof to improving cold resistance of plants

A cold resistance, tea tree technology, applied in the field of genetic engineering, can solve problems that are rarely reported

Active Publication Date: 2021-07-23
ANHUI AGRICULTURAL UNIVERSITY
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  • Claims
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Problems solved by technology

In summary, WRKY transcription factors are widely involved in plant biotic and abiotic stress responses, and play an impo...

Method used

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  • Camellia sinensis(L.)O.Kuntze) WRKY29 gene and application thereof to improving cold resistance of plants
  • Camellia sinensis(L.)O.Kuntze) WRKY29 gene and application thereof to improving cold resistance of plants
  • Camellia sinensis(L.)O.Kuntze) WRKY29 gene and application thereof to improving cold resistance of plants

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Embodiment 1

[0020] 1. Cloning and sequence structure analysis of CsWRKY29 gene

[0021] Tea tree WRKY29 gene (i.e. CsWRKY29 gene) is a tea tree WRKY class transcription factor gene, and its cloning and sequence structure analysis are as follows:

[0022] Shucha, a national-level tea tree variety, was first planted in the Agricultural Industrial Park of Anhui Agricultural University, Hefei, Luyang District, Anhui Province. The young leaves were used for RNA extraction. Total RNA was extracted using RNAprep Pure Plant Kit (Tiangen, Beijing, China) according to the instructions, and the RNA content and quality were detected with a spectrophotometer.

[0023] Reverse transcription to generate the first strand: take 1 μg RNA as template, configure according to PrimeScript II 1st StrandcDNASynthesis Kit (Takara Biotech, China) kit instructions, add Oligo dT Primer (50 μM) 0.6 μl, Random 6mers (50 μM) 0.4 μl, dNTP Mixture (10mM each) 1μl, RNase Free dH 2 Make up to 10 μl with O, denature at 65...

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Abstract

The invention belongs to the technical field of gene engineering, and discloses a camellia sinensis(L.)O.Kuntze) WRKY29 gene and an application thereof to improving cold resistance of plants. The nucleotide sequence of the camellia sinensis(L.)O.Kuntze) WRKY29 gene is shown as SEQ ID NO.1 in a sequence table. The amino acid sequence of the protein coded by the camellia sinensis(L.)O.Kuntze) WRKY29 gene is shown as SEQ ID NO.2 in the sequence table. The expression mode of the camellia sinensis(L.)O.Kuntze) WRKY29 is related to the response of camellia sinensis(L.)O.Kuntze) to low-temperature stress, the gene is over-expressed to transform plants, the cold resistance of the plants can be improved, the expression of CsWRKY29 is inhibited through an antisense oligonucleotide technology, and the damage of camellia sinensis(L.)O.Kuntze) leaves at low temperature is obviously aggravated. The cloning of the gene not only is beneficial to exploring the effect of a WRKY transcription factor in the cold resistance process of the camellia sinensis(L.)O.Kuntze), but also is beneficial to promoting the genetic improvement process aiming at enhancing the cold resistance of the camellia sinensis(L.)O.Kuntze) and promoting the sustainable development of the tea industry, so that the gene has a great application value.

Description

technical field [0001] The invention relates to the technical field of genetic engineering, in particular to the tea tree WRKY29 gene and its application in improving plant cold resistance. Background technique [0002] Tea tree (Camellia sinensis (L.) O. Kuntze) is an important economic crop in my country. Tea leaves are rich in amino acids, alkaloids, tea polysaccharides, polyphenols, and volatile substances. The content and composition of these inclusions are different, which together endow tea with quality characteristics such as color, aroma, and taste. China's tea regions are mainly divided into Southwest tea regions, South China tea regions, Jiangnan tea regions, and Jiangbei tea regions, and there are few distributions in the northern regions. In recent years, tea trees in overwintering and early spring frequently suffer from low temperature damage such as "frost" or "cold spring", and the new tea trees are slightly damaged, affecting the yield and quality of tea, a...

Claims

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Application Information

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IPC IPC(8): C12N15/29C12N15/82C07K14/415A01H5/00A01H6/00
CPCC07K14/415C12N15/8273
Inventor 夏恩华赵慧娟童伟吴琼
Owner ANHUI AGRICULTURAL UNIVERSITY
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