Nitrogen-Regulated Sugar Sensing Gene and Protein and Modulation Thereof

a sugar sensing gene and nitrogen-regulated technology, applied in the direction of sugar derivatives, plant cells, angiosperms/flowering plants, etc., can solve the problems of increasing the cost of this input to the farmer, increasing the cost of this input to the environment, and reducing the effect of resistance to herbicides, enhancing or reducing the requirement for light, water, nitrogen or trace elements

Inactive Publication Date: 2007-10-25
GUELPH UNIV OF
View PDF4 Cites 15 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0193] Embodiments of the present invention also relate to a plant modified by a method that includes introducing into a plant a nucleic acid where the nucleic acid is expressible in the plant in an amount effective to effect the modification. The modification can be, for example, carbon, nitrogen and/or sulfur metabolism, nitrogen utilization, nitrogen assimilation, photosynthesis, yield, chlorophyll synthesis, signal transduction, cell growth, reproduction, disease resistance, abiotic stress tolerance, nutritional composition, gene regulation, and/or differentiation. In one embodiment, the modified plant has increased or decreased resi...

Problems solved by technology

However, the use of large amounts of nitrogen fertilizer has negative side-effects primarily around increasing cost of this input to the farmer and cost to the environment since nitrate pollution is a major problem in many agricultural areas contributing significantly to the degradation of both fresh water and marine environments.
These experiments have demonstrated that there is a genetic component to nitrogen use efficiency, but have not proved satisfactory in determining which genes are important for this process.
In addition, corn breeders have generally not targeted the maintenance of yield under limiting nitrogen fertilizer...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Nitrogen-Regulated Sugar Sensing Gene and Protein and Modulation Thereof
  • Nitrogen-Regulated Sugar Sensing Gene and Protein and Modulation Thereof
  • Nitrogen-Regulated Sugar Sensing Gene and Protein and Modulation Thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

Cloning and Sequence of At5g56860

[0420] Gene predictions were derived from the sequence databases, and used to design oligonucleotide primers for PCR amplification of either full-length (inclusive of the predicted initiation and stop codons, for transgenic gene overexpression) or partial (for transgenic gene knockout) cDNA clones from rice first strand cDNA. In some instances, these PCR primers included additional 5′ sequences for Gateway™ recombination-based cloning (Invitrogen). PCR amplification was carried out using the HF Advantage II (Clonetech) or EXPAND (Roche) PCR kits according to the manufacturer's instructions. PCR products were cloned into pCR2.1-TOPO or pDONR201 according to the manufacturer's instructions (Invitrogen).

[0421] DNAs from 4-8 independent clones were miniprepped following the manufacturer's instructions. DNA was subjected to sequencing analysis using the BigDye™ Terminator Kit according to manufacturer's instructions (ABI). Sequencing made use of primer...

example 2

[0441] The full length At5g56860 cDNA (GNC) was amplified from Arabidopsis leaf cDNA using the primers 5′-GCTCTAGATTTCTCTCTCTCTTTGTGTCTTCATTTG-3′ (SEQ ID NO:4) and 5′-gcgagctctcgggtgactaatgttcgttcc-3′ (SEQ ID NO:5). The resulting ˜1500 bp fragment was verified by sequencing and then digested by XbaI and SacI and cloned into the XbaI-SacI-digested expression vector pROK2 containing the cauliflower mosaic virus (CaMV) 35S promoter driving the GNC expression in a constitutive, high-level fashion (FIG. 4). The p35S-GNC binary vector was transformed into Agrobacterium tumefaciens strain EHA105 and the resulting Agrobacterium strain was used to transform the wild type plants (Col-0) and the transformants were selected on kanamycin resistance. Plants with a single insertion of the transgene were selected for self pollination to generate T3 homozygous lines for further analysis. Over-expression of GNC in the transgenics was confirmed by quantitative RT-PCR. Wild type plants and GNC over-exp...

example 3

[0442] As mentioned in Example 1, the inventors identified the Arabidopsis GATA transcription factor gene GNC (At5g56860) important in chlorophyll synthesis and sugar sensitivity. The At4g26150 gene is a GNC paralog in the phylogenetic tree of the 30 Arabidopsis GATA transcription factor genes (Reyes, J. C., Muro-Pastor, M. I. & Florencio, F. J. (2004) Plant Physiol. 134, 1718-1732) and was found to have overlapping function with GNC (unpublished results). In the rice (Oryza sativa) genome, there are 28 GATA transcription factor genes, with one pair of genes (OsGATA16 and OsGATA11) sharing similarity with the two Arabidopsis GATA genes (Reyes, J. C., Muro-Pastor, M. I. & Florencio, F. J. (2004) Plant Physiol. 134, 1718-1732). Transgenic rice plants either over-expressing or silencing the two rice ortholog genes were generated. The phenotypes of these transgenic plants were analyzed to understand their in vivo function.

Materials and Methods

Plant Growth Conditions

[0443] Peat moss...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Nucleic acid sequenceaaaaaaaaaa
Energyaaaaaaaaaa
Stress optical coefficientaaaaaaaaaa
Login to view more

Abstract

The present invention relates to a nitrogen-regulated GATA transcription factor gene required for sugar sensing and the modulation of the expression of this gene to modulate a characteristic in a plant. The GATA transcription factor of the present invention is involved in regulating sugar sensing in plants and its expression is influenced by nitrogen status. Increased expression of this or substantially similar genes can produce plants with improved nitrogen utilization and increased yield.

Description

[0001] This application is a continuation-in-part of U.S. patent application Ser. No. 11 / 331,199 filed Jan. 13, 2006 which claims priority from U.S. provisional patent application No. 60 / 643,575 filed Jan. 14, 2005, which are incorporated herein by reference in their entirety.FIELD OF THE INVENTION [0002] The present invention relates to methods of modulating agronomic traits in plants by modulating the expression of a GATA transcription factor in the plant cells. In particular the present invention relates to methods of improving nitrogen utilization in plants. The present invention also pertains to nucleic acid molecules isolated from Arabidopsis thaliana comprising nucleotide sequences that encode proteins that are sugar sensing and, ultimately, can modulate nitrogen uptake and overall carbon metabolism. BACKGROUND OF THE INVENTION [0003] Improvement of the agronomic characteristics of crop plants has been ongoing since the beginning of agriculture. Most of the land suitable for ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H1/00A01H5/00C07H19/00C07K16/00C12N5/04
CPCC07K14/415C12N15/8242C12N15/8261C12N15/8245C12N15/8243Y02A40/146
Inventor ROTHSTEIN, STEVENBI, YONG-MEI
Owner GUELPH UNIV OF
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap