Maize water-logging tolerance-related transcription factor gene zm-bRLZ, molecular marker and application

A technology of transcription factor and water tolerance, applied in the field of plant biology, can solve the problems of enhancing long-term adaptive response of Arabidopsis thaliana and the premature death of transformed plants.
CN101974537AInactive Publication Date: 2011-02-16HUAZHONG AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
HUAZHONG AGRI UNIV
Publication Date
2011-02-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the technical field of plant gene engineering, and relates to clone and application of a maize water-logging tolerance-related transcription factor gene zm-bRLZ. The nucleotide sequence of the gene is shown as SEQ ID No. 1, and the gene has a total length of 4,027bp and comprises 6 exons. The cDNA sequence of the gene is shown as SEQ ID No. 2, and 282 amino acids are encoded on the gene. In flooding stress, the expression of the gene in maize inbred line Hz32 seedling roots is up-regulated and the expression level of the gene in the Mo17 is kept unchanged. The in-vitro combination of a gene protein product and an antidiuretic hormone (ADH) promoter anaerobic response factor shows that the zm-bRLZ gene has a regulation and control effect on an anaerobic induced gene. A pair of cleaved amplified polymorphic sequence (CAPS) markers is developed by utilizing zm-bRLZ gene sequence difference of the maize water-logging tolerance inbred line Hz32 and high-sensitivity K12. The gene is positioned at a water-logging tolerance quantitative trait locus (QTL) peak part of 9.04bin by utilizing K12*Hz32 F2 group. A candidate gene correlation analysis proves that a zm-bRLZ gene promoter region and a plurality of single nucleotide polymorphism (SNP) sites at a 3'-untranslated region (3'-UTR) are all obviously associated with a plurality of water-logging tolerance indexes.
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Description

technical field

[0001] The invention relates to the field of plant biotechnology. It specifically relates to the cloning of a transcription factor gene zm-bRLZ, which is located on the 9th chromosome of maize and is related to waterlogging tolerance of maize seedlings, and the development and application of its functional markers. Background technique

[0002] Spring corn in the south and summer corn in the north often suffer from floods and waterlogging during the normal cultivation season. Long-term waterlogging makes the corn root system in a low-oxygen state, resulting in a reduction in corn yield. Waterlogging has become an important abiotic stress factor restricting the high and stable yield of maize. Breeding waterlogging-tolerant maize varieties is an effective way to reduce losses caused by flooding stress.

[0003] Many studies have shown that there are genetic differences in waterlogging tolerance among maize inbred lines, which provide genetic resources for gen...

Claims

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