Cosmetic substrate containing double-bacterium fermentation soy milk extract and application
A technology of cosmetic matrix and extract, which is applied in the direction of medical preparations, cosmetics, and cosmetic preparations containing active ingredients. Significant effect of aging effect
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Embodiment 1
[0034] Embodiment 1 (preparation of soymilk extract prepared by dual bacterial fermentation)
[0035] A preparation process for preparing soybean milk extract by dual-bacteria fermentation, the specific steps are:
[0036] After soaking, adjust the soybean water ratio to 1:8, make pulp, and sterilize; add 1% sucrose (after sterilization) as a starter starter; mix Lactobacillus bulgaricus and Saccharomyces cerevisiae at a mass ratio of 1:1 (the total inoculum size is 4%) into the cooled sterilized soymilk, first cultured at 37°C for 8h, then transferred to 30°C, 200rpm shaker for 48h; centrifuged at 8000rpm for 5min to remove insoluble matter such as bacteria and bean dregs.
[0037] The content changes of functional components such as daidzein (Daidzein), genistein (Genistein) and glycitein (Glycitein) in soybean milk samples before and after fermentation were detected by HPLC, as shown in Table 1.
[0038] Table 1 Changes (%) of functional components of soy milk (SOY) and ...
Embodiment 2
[0043] Embodiment 2 (dual bacterial fermentation prepares the preparation of soybean milk extract)
[0044] A preparation process for preparing soybean milk extract by dual-bacteria fermentation, the specific steps are:
[0045] Slurry soybeans, peeled potatoes and water at a ratio of 10:1:89, and sterilize; add Lactobacillus rhamnosus and Pichia pastoris with a mass ratio of 1:2 to the cooled soybean milk (the total inoculum amount is 2 %), 35°C, 200rpm shaker culture until 60h; filter to remove insoluble matter such as bacteria and bean dregs; use an ultrafiltration membrane with a molecular weight cut-off of 3kDa to separate macromolecular proteins.
Embodiment 3
[0046] Example 3 (preparation of cosmetic matrix containing soybean milk extract prepared by double bacteria fermentation)
[0047] A method for preparing a cosmetic matrix with remarkable instant brightening effect and moisturizing and long-acting anti-wrinkle effects, the specific steps are as follows:
[0048] ①Start stirring (rotating speed: 800rpm), at 50°C, slowly add 1% sodium oligomeric hyaluronate into 24% deionized water, and stir at constant temperature for 30 minutes to obtain sodium oligomeric hyaluronate solution;
[0049] ② Add 45% fermented soybean milk extract and 30% fungus polysaccharide (this product comes from Xi'an Tianrui Biotechnology Co., Ltd.) to the oligomeric sodium hyaluronate solution in sequence, stir for 5 minutes, and then homogenize at a low speed (the homogenization speed is 1500rpm) for 3min, cooled to 20°C, and discharged to obtain a skin care base with moisturizing and long-acting anti-wrinkle effects.
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