Preparation method of auricularia auricula polysaccharide

A technology of fungus polysaccharides and black fungus, applied in metabolic diseases, drug combinations, etc., can solve the problems of poor water solubility of products, loss of efficacy of active substances, low extraction rate of black fungus polysaccharides, etc., and achieve enhanced blood lipid-lowering effect and enhanced blood-lipid-lowering activity , good crushing effect

Active Publication Date: 2017-09-19
INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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Problems solved by technology

[0007] In order to solve technical problems such as the low extraction rate of black fungus polysaccharides in the prior art, the serious loss of active substances in the extraction process, and the poor water solubility of the obtained products, the present invention provides a preparation method and application of black fungus polysaccharides. The adopted technology The scheme is as follows:

Method used

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  • Preparation method of auricularia auricula polysaccharide
  • Preparation method of auricularia auricula polysaccharide
  • Preparation method of auricularia auricula polysaccharide

Examples

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Embodiment 1

[0030] Embodiment 1: the preparation method of a kind of fungus polysaccharide in the present embodiment is carried out according to the following steps:

[0031] Step 1. Add the black fungus powder to a citric acid-sodium citrate buffer solution with a pH value of 5.0 and a concentration of 0.1mol / L. The amount of the buffer solution is 40 times the volume of the black fungus powder, and stir evenly to obtain a black fungus suspension liquid;

[0032] Wherein, the black fungus powder is obtained by crushing the black fungus and passing through a 800 mesh sieve;

[0033] Step 2, then add pectinase with an enzyme activity of 15000U / g, the amount of pectinase added is 4% (m / v) of the black fungus suspension, and enzymolyze it in a water bath at 50°C for 90min to obtain an enzymolyzed solution A;

[0034] Step 3, add cellulase with an enzyme activity of 15000 U / g, the amount of cellulase added is 2% (m / v) of the enzymolysis solution A, and enzymolyze it in a water bath at 50°C ...

Embodiment 2

[0096] Example 2: The difference between this example and Example 1 is that the enzymolysis temperature of neutral protease is 50°C, the enzymolysis temperature of the other two enzymes is 45°C, and the enzymolysis time is 120min. Other steps and parameters Same as Example 1. After testing, the yield of the obtained black fungus polysaccharide is 48.1%, and the solubility is 99.79%.

Embodiment 3

[0097] Example 3: The difference between this example and Example 1 is that this embodiment provides a method for extracting black fungus polysaccharides, and the technical solution adopted is different from Example 1 in that: the enzymatic hydrolysis of neutral protease The temperature is 50° C., the enzymolysis temperature of the other two enzymes is 55° C., and the enzymolysis time is 120 minutes. Other steps and parameters are the same as in Example 1. After testing, the yield of the obtained black fungus polysaccharide is 47.8%, and the solubility is 99.38%.

[0098] Embodiment 3: This embodiment differs from Embodiment 1 in that the ultrasonic treatment conditions are: ultrasonic power 300W, processing time 40 min, and other steps and parameters are the same as in Embodiment 1. After testing, the obtained black fungus polysaccharide has a yield of 47.9% and a solubility of 99.67%.

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Abstract

The invention discloses a preparation method of auricularia auricula polysaccharide and belongs to the technical field of active ingredient extraction of edible fungi. The preparation method comprises steps as follows: auricularia auricular is smashed, dissolved and subjected to enzymolysis with pectinase, cellulase and alpha-amylase in sequence, then pH is regulated, enzymolysis is performed with neutral protease, ultrasonic treatment is performed after enzymolysis, extraction treatment is performed after ultrasonic treatment, a clear liquid is separated after extraction, and auricularia auricula polysaccharide is obtained after concentration, alcohol precipitation and drying. In consideration of factors such as extraction process cycle, ecological protection, energy consumption, production cost, product activity, process complexity and the like, the preparation method has the advantages of high efficiency, good crushing effect and high yield, and blood lipid reducing activity of extract is greatly enhanced. According to the method for preparing the auricularia auricular extract, the yield is up to 45% or higher, the blood lipid reducing effect of the obtained auricularia auricular polysaccharide is greatly enhanced, various indexes of blood lipid reach significant difference level, (p is smaller than 0.01), and the preparation method is obviously better than existing and conventional preparation methods.

Description

technical field [0001] The invention belongs to the technical field of extracting active components of edible fungi, and in particular relates to a preparation method of fungus polysaccharide. Background technique [0002] Black fungus (Auricularia auricula) is a well-known medicinal and edible fungus in my country. It has high nutritional and medicinal value. It has a large output and good quality in the three northeastern provinces, and its industry has developed rapidly. Black fungus has biological activities such as lowering blood fat, antithrombotic, anti-radiation, anti-mutation, anti-oxidation, anti-aging, lowering blood sugar, improving immunity, and anti-cancer. In recent years, it has been clarified through research that high blood pressure and high blood fat are the main reasons for high levels of cardiovascular and cerebrovascular diseases, which can directly lead to cardiovascular and cerebrovascular diseases such as cerebral infarction, fatty liver, cholelithia...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P3/06
CPCC08B37/0003
Inventor 孔祥辉张介驰马银鹏于德水王玉霞赵金海张丕奇戴肖东王笑庸陈鹤
Owner INST OF MICROBIOLOGY HEILONGJIANG ACADEMY OF SCI
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