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Preparation method of auricularia auricular polysaccharides

A technology of fungus polysaccharides and fungus, which is applied in the field of food processing and can solve problems such as no discovery

Active Publication Date: 2012-12-19
LONGHAI YINGXIN AGRI TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

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Method used

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Embodiment 1

[0031] 1. a preparation method of fungus polysaccharide, the method may further comprise the steps:

[0032] (1) Raw material pretreatment

[0033] Soak the dried fungus in water for 12 hours;

[0034] (2) High temperature cooking

[0035] The treated fungus was added to water for high-temperature cooking, the cooking temperature was 100°C, and the cooking time was 45 minutes to obtain a crude fungus polysaccharide extract, wherein the weight ratio of fungus to water was 1:10;

[0036] (3) Preparation of two-phase aqueous system

[0037] The total mass of the two-phase aqueous system is 10.0 g, which contains a polyethylene glycol solution with a mass fraction of 15% and a relative molecular mass of 4000 and ammonium sulfate with a mass fraction of 14%. The two-phase aqueous system is shaken and mixed, and left to stand Phase;

[0038] (4) The crude fungus polysaccharide extract was filtered and added to the above-mentioned two-phase system for centrifugation, and the phas...

Embodiment 2

[0041] 1. a preparation method of fungus polysaccharide, the method may further comprise the steps:

[0042] (1) Raw material pretreatment

[0043] Soak the dried fungus in clean water for 12.5 hours;

[0044] (2) High temperature cooking

[0045] The treated fungus was added to water for high-temperature cooking, the cooking temperature was 105°C, and the cooking time was 47 minutes to obtain a crude fungus polysaccharide extract, wherein the weight ratio of fungus to water was 1:11;

[0046] (3) Preparation of two-phase aqueous system

[0047] The total mass of the two-phase aqueous system is 10.0 g, which contains a polyethylene glycol solution with a mass fraction of 16% and a relative molecular mass of 5000 and ammonium sulfate with a mass fraction of 15%. The two-phase aqueous system is shaken and mixed, and left to stand Phase;

[0048] (4) After filtering the crude fungus polysaccharide extract, add it to the above-mentioned two-phase system for centrifugation, and...

Embodiment 3

[0051] 1. a preparation method of fungus polysaccharide, is characterized in that, the method comprises the following steps:

[0052] (1) Raw material pretreatment

[0053] Soak the dried fungus in water for 13 hours;

[0054] (2) High temperature cooking

[0055] The treated fungus was added to water for high-temperature cooking, the cooking temperature was 110°C, and the cooking time was 50 minutes to obtain a crude fungus polysaccharide extract, wherein the weight ratio of fungus to water was 1:12;

[0056] (3) Preparation of two-phase aqueous system

[0057] The total mass of the two-phase aqueous system is 10.0 g, which contains a polyethylene glycol solution with a mass fraction of 25% and a relative molecular mass of 6000 and ammonium sulfate with a mass fraction of 17%. The two-phase aqueous system is shaken and mixed, and left to stand Phase;

[0058] (4) The crude fungus polysaccharide extract was filtered and added to the above-mentioned two-phase system for cen...

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Abstract

The invention discloses a preparation method of auricularia auricular polysaccharides. The method adopts agarics as raw materials, and extracts auricularia auricular polysaccharides with a method for combining the polyethylene glycol / ammonium sulfate two aqueous phase extraction and the hollow fiber membrane separation by stewing at high temperature; and the extracted polysaccharides are spray-dried to obtain the solid powder auricularia auricular polysaccharides. The method can obtain high-purity soluble auricularia auricular polysaccharides. Meanwhile, the method can combines the membrane separation with the two aqueous phase extraction, thus greatly fastening the extraction mass transfer rate. The two aqueous phase system is separated by the membranes so as to avoid the emulsification resulted from small interfacial tension of the two aqueous phase system, and greatly reduce the dosage of organic solvents.

Description

technical field [0001] The invention belongs to the field of food processing, and in particular relates to a preparation method of fungus polysaccharide. Background technique [0002] Fungus contains acidic mucopolysaccharides, which are composed of monosaccharides such as L-fucose, L-arabinose, D-xylose, D-mannose, D-glucose and glucuronic acid, and are gray-white filamentous. Fungus polysaccharides have a preventive effect on hyperglycemia. It can significantly reduce the formation of hypercholesterolemia caused by hyperlipidemia and high cholesterol; fungus polysaccharides can promote the immune function of the body, including increasing spleen index, half-fold hemolysis value and E rosette formation rate. Promote macrophage phagocytosis and lymphocyte transformation rate, enhance the body's cellular immunity and humoral immunity, and have significant anti-tumor activity; fungus polysaccharides have anti-aging activity, enhance nucleic acid and protein metabolism. It ca...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 黄建实
Owner LONGHAI YINGXIN AGRI TECH
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