Method for extracting polysaccharide from auricularia
A fungus polysaccharide and fungus technology, which is applied in the field of extracting fungus polysaccharides, can solve problems such as coarseness, reduced bioavailability, easy to destroy polysaccharide structure, etc., and achieves the effects of simple operation, high polysaccharide yield and proficiency, and good product safety.
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Embodiment 1
[0019] A method for extracting polysaccharides from fungus, through the following process steps:
[0020] A, raw material selection, select ripe, dry fungus without disease and pest as raw material;
[0021] b, raw material processing, pulverizing the fungus raw material selected in step a;
[0022] c. Add water 7 times the mass of the crushed fungus in step b, stir evenly, and soak for 3.5 hours to obtain the fungus slurry;
[0023] d. During the compound enzyme heat preservation enzymolysis process, add an appropriate amount of water to the fungus slurry obtained in step c, stir evenly, control the enzyme heat preservation temperature to 50°C, adjust the pH value to 5.0, and add cellulase with a liquid solid content of 0.3%. Carry out enzymatic hydrolysis, and the enzymatic hydrolysis time is 80min;
[0024] e. Enzyme-inactivated leaching; after enzymatic hydrolysis, heat up to 100°C and keep for 30min;
[0025] f, centrifugation, transfer the solid-liquid mixture of fung...
Embodiment 2
[0031] A method for extracting polysaccharides from fungus, through the following process steps:
[0032] A, raw material selection, select ripe, dry fungus without disease and pest as raw material;
[0033] b, raw material processing, pulverizing the fungus raw material selected in step a;
[0034] c. Add water 5 to 10 times the mass of the crushed fungus in step b, stir evenly, and soak for 4 hours to obtain the fungus slurry;
[0035] d. During the compound enzyme heat preservation enzymolysis process, add an appropriate amount of water to the fungus slurry obtained in step c, stir evenly, control the enzyme heat preservation temperature to 45°C, adjust the pH value to 5.0, and add cellulase with a liquid solid content of 0.5% Carry out enzymatic hydrolysis, and the enzymatic hydrolysis time is 80min;
[0036] e. Enzyme-inactivated leaching; after enzymatic hydrolysis, heat up to 100°C and keep for 30min;
[0037] f, centrifugation, transfer the solid-liquid mixture of f...
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