Method for extracting polysaccharide from auricularia

A fungus polysaccharide and fungus technology, which is applied in the field of extracting fungus polysaccharides, can solve problems such as coarseness, reduced bioavailability, easy to destroy polysaccharide structure, etc., and achieves the effects of simple operation, high polysaccharide yield and proficiency, and good product safety.

Inactive Publication Date: 2017-05-31
昭平县科学技术指导站
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The fungus cells are thick and the wall is thick, so it is difficult to dissolve the polysaccharides from the water extraction; and the acid-base extraction method is easy to destroy the polysaccharide structure and reduce its bioavailability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A method for extracting polysaccharides from fungus, through the following process steps:

[0020] A, raw material selection, select ripe, dry fungus without disease and pest as raw material;

[0021] b, raw material processing, pulverizing the fungus raw material selected in step a;

[0022] c. Add water 7 times the mass of the crushed fungus in step b, stir evenly, and soak for 3.5 hours to obtain the fungus slurry;

[0023] d. During the compound enzyme heat preservation enzymolysis process, add an appropriate amount of water to the fungus slurry obtained in step c, stir evenly, control the enzyme heat preservation temperature to 50°C, adjust the pH value to 5.0, and add cellulase with a liquid solid content of 0.3%. Carry out enzymatic hydrolysis, and the enzymatic hydrolysis time is 80min;

[0024] e. Enzyme-inactivated leaching; after enzymatic hydrolysis, heat up to 100°C and keep for 30min;

[0025] f, centrifugation, transfer the solid-liquid mixture of fung...

Embodiment 2

[0031] A method for extracting polysaccharides from fungus, through the following process steps:

[0032] A, raw material selection, select ripe, dry fungus without disease and pest as raw material;

[0033] b, raw material processing, pulverizing the fungus raw material selected in step a;

[0034] c. Add water 5 to 10 times the mass of the crushed fungus in step b, stir evenly, and soak for 4 hours to obtain the fungus slurry;

[0035] d. During the compound enzyme heat preservation enzymolysis process, add an appropriate amount of water to the fungus slurry obtained in step c, stir evenly, control the enzyme heat preservation temperature to 45°C, adjust the pH value to 5.0, and add cellulase with a liquid solid content of 0.5% Carry out enzymatic hydrolysis, and the enzymatic hydrolysis time is 80min;

[0036] e. Enzyme-inactivated leaching; after enzymatic hydrolysis, heat up to 100°C and keep for 30min;

[0037] f, centrifugation, transfer the solid-liquid mixture of f...

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PUM

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Abstract

The invention discloses a method for extracting polysaccharide from auricularia. The method comprises the following steps of selecting dried auricularia as a raw material, soaking, cleaning, crushing, adding cellulase, controlling enzymolysis conditions, and hydrolyzing by enzyme, thus breaking down cell walls; extracting auricularia residues, subjected to enzyme hydrolysis, by hot water; combining an auricularia enzyme hydrolysis solution and an auricularia extracting solution, filtering, adding a tannic acid solution, removing protein out of filtrate, and performing alcohol settling on a centrifugally collected polysaccharide solution by an ethanol solution; finally, vacuum-drying to obtain a polysaccharide product. The extraction method has the advantages that a technology is reasonable, the operation is simple, components are comprehensively reserved, the a yield rate and maturity of the polysaccharide are high, and the safety of the product is good.

Description

technical field [0001] The invention relates to the field of polysaccharide preparation technology, and more specifically relates to a method for extracting polysaccharides from fungus. Background technique [0002] We know that fungus belongs to Basidiomycetes, Auricularia and Agaricaceae in mycological classification. The color is dark brown, the texture is soft, and it can prevent iron deficiency anemia and other medicinal effects. Mainly distributed in Heilongjiang, Jilin, Fujian, Taiwan, Hubei, Guangdong, Guangxi, Sichuan, Guizhou, Yunnan and other places. The fruiting bodies of fungus contain acidic mucopolysaccharides, which are composed of monosaccharides such as L-fucose, L-arabinose, D-xylose, D-mannose, D-glucose and glucuronic acid. The fungus polysaccharide extracted from the fungus has the effects of lowering blood sugar, blood fat, lowering cholesterol, inhibiting platelet aggregation, improving the immune function of the body, anti-thrombosis, anti-aging, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/0024
Inventor 李荣江
Owner 昭平县科学技术指导站
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