Preparation method of auricularia polysaccharide with hypoglycemic effect

A technology of black fungus polysaccharides and black fungus, which can be used in metabolic diseases, drug combinations, etc. It can solve the problems of high viscosity, unfavorable fluidity and diffusion of enzyme molecules, increase the difficulty of the extraction and preparation process, increase the viscosity of the extraction system, etc., and achieve ideal particle size distribution. , good fluidity, little damage to activity

Active Publication Date: 2015-09-02
CHINA JILIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, black fungus is a special colloidal fungus, which is different from common edible fungi (such as mushrooms), and its carbohydrate content is very high. After the black fungus absorbs water and swells, the soluble part flows out from the tissue , which increases the viscosity of the extraction system, which increases the difficulty of the extraction and preparation process of black fungus polysaccharides, especially for the preparation method that needs to use enzyme catalysis in the preparation process. High viscosity is not conducive to the fluidity and diffusion of enzyme molecules

Method used

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  • Preparation method of auricularia polysaccharide with hypoglycemic effect
  • Preparation method of auricularia polysaccharide with hypoglycemic effect
  • Preparation method of auricularia polysaccharide with hypoglycemic effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Materials and reagents

[0039] Black fungus (provided by Hangzhou Huadan Agricultural Products Co., Ltd.); pNPG, purchased from sigma; α-glucosidase, purchased from Jiangsu Ruiyang Biological Co., Ltd.; saliva; pepsin; pancreatin; hydrochloric acid, potassium dihydrogen phosphate, hydrogen Sodium oxide, phenol, concentrated sulfuric acid and other reagents were of analytical grade.

[0040] 2. Experimental equipment

[0041] UV-2000 ultraviolet spectrophotometer; DFY-400 pulverizer; SANYO ultra-low temperature refrigerator; RE-52A rotary evaporator; JJ series high-pressure homogenizer; JP-250A-I high-speed multifunctional pulverizer; L04 electronic balance; DELTA -320 pH meter; 85-Z magnetic stirrer; HH-2 water bath; DHG-9240A blast drying oven; (glucose oxidase method).

[0042] 3. Experimental method

[0043] (1) Black fungus pretreatment

[0044] Take 100g of dried black fungus fruiting bodies, coarsely grind them mechanically, and pass through a 60-mesh sie...

Embodiment 2

[0119] The experimental operation is the same as that in Example 1, that is, take 100 g of dried black fungus fruiting bodies, coarsely crush them mechanically, and pass through a 60-mesh sieve to obtain black fungus powder. Add the obtained black fungus powder to 1000 mL of deionized water, fully swell, and store at -80°C at ultra-low temperature Freeze under the environment, then mechanically roughly separate, then further use a high-pressure homogenizer, under the condition of a homogenizing pressure of 12MPa, homogenize for 6 minutes, and obtain 967g of black fungus superfine pulverized sauce. The difference is that the black fungus fruiting bodies were frozen at -80°C for 12 hours at an ultra-low temperature, and other operations and conditions were still the same as in Example 1 to obtain 14.76 g of the product, wherein the black fungus polysaccharide content was 62.84%, and the dissolution rate of the black fungus polysaccharide was 14.76%. The inhibition rate of α-gluco...

Embodiment 3

[0121] The experimental operation is the same as that in Example 1, that is, take 100 g of dried black fungus fruiting bodies, coarsely crush them mechanically, and pass through a 60-mesh sieve to obtain black fungus powder. Add the obtained black fungus powder to 1000 mL of deionized water, fully swell, and store at -80°C at ultra-low temperature Freeze under the environment, then mechanically roughly separate, then further use a high-pressure homogenizer, under the condition of a homogenizing pressure of 12MPa, homogenize for 6 minutes, and obtain 967g of black fungus superfine pulverized sauce. The difference is that the black fungus fruit body was frozen at -80°C for 24 hours at ultra-low temperature, and other operations and conditions were still the same as in Example 1 to obtain 16.31 g of the product, wherein the black fungus polysaccharide content was 63.75%, and the dissolution rate of the black fungus polysaccharide was 16.31%. The inhibition rate of α-glucosidase wa...

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Abstract

The invention discloses an extraction method of auricularia polysaccharide. The method comprises the following steps: carrying out mechanical coarse grinding on dried fruiting body of auricularia auricular, mixing the ground fruiting body of auricularia auricular with deionized water and fully swelling, freeze-drying, carrying out mechanical rough segmentation, homogenizing by a high pressure homogenizer, fully oscillating and mixing the auricularia auricular ultrafine-ground paste and fresh saliva, adding deionized water, adjusting pH to 1.7-1.9, adding artificial gastric juice, stirring, carrying out water-bath insulation, maintaining pH of the system to 6.6-7.0, adding a pancreatin solution, stirring, carrying out water-bath insulation, warming up and carrying out enzyme deactivation, centrifuging a mixed liquor, taking a supernatant, adding a mixture of diatomite and active carbon, fully stirring and filtering, taking a filtrate, carrying out membrane filtration by the use of a polysulfone membrane, collecting a small molecular solution, and drying to obtain auricularia polysaccharide. By the ultralow-temperature homogenization technology and by synergism of saliva and artificial gastric juice, hypoglycemic activity of polysaccharide is ensured when polysaccharide is fully dissolved out, and polysaccharide obtained has high purity and less damage to activity.

Description

(1) Technical field [0001] The invention relates to a preparation method of black fungus polysaccharide with hypoglycemic effect. (2) Background technology [0002] Black fungus [Auricularia auricular (Hook.) Underw, referred to as AA] belongs to Basidiomycotina, Hymenomycetes, Auriculares, Auricularaceae, and Auricularia. my country is one of the main countries producing black fungus in the world, accounting for 90% of the total output of black fungus in the world. [0003] The carbohydrates in black fungus account for about 70% of its dry weight. In addition to mannan, dextran, acid polysaccharides, chitin, cellulose and other substances, it also contains some trace components, such as melanin and phenols. compounds etc. Black fungus polysaccharide, as a natural carbohydrate with various biological activities, has been increasing in recent years about its hypoglycemic aspects, such as Xue Weijian et al. (Journal of China Pharmaceutical University, 1989,20(3):181-183) Fu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P3/10
Inventor 张拥军徐思绮王爽宋广磊朱丽云
Owner CHINA JILIANG UNIV
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