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Development and application of antibody gene chip

A gene chip and gene chip hybridization technology, which is applied in the development and application of antibody gene chips, can solve the problems of low sensitivity, expensive, and inability to detect tissue slices, and achieve the effect of low laboratory conditions and cost reduction

Inactive Publication Date: 2013-10-30
朱有凯
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Problems solved by technology

These techniques are very expensive, the relevant reagents are not easy to store, and the detection time is long, etc.; the detection sensitivity is low, and it is difficult to detect low-abundance proteins
These conditions lead to proteome research techniques and results can not be directly applied to the clinical diagnosis and treatment process
In addition, all current proteome research techniques cannot be directly used for the detection of tissue sections

Method used

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  • Development and application of antibody gene chip
  • Development and application of antibody gene chip
  • Development and application of antibody gene chip

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Experimental program
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Embodiment Construction

[0019] The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0020] exist figure 1 In this method, monoclonal antibodies are screened from the phage antibody library to form a specific antibody library (containing 20-50 antibodies), and the V-D-J fragments of each antibody are respectively amplified with universal primers or specific primers, and purified as probes to prepare each Antibody gene chip of antibody V-D-J fragment.

[0021] exist figure 2 In the process, the specific antibody library is added to the detection object (such as tissue slices), after washing, E. coli TG1 is added to incubate, TG1 is expanded and cultured to extract the antibody gene, the PCR amplified antibody V-D-J fragment is hybridized with the antibody gene V-D-J fragment chip, and the interpretation of the hybridization result shows the detection Protein expression profiling.

[0022] exist Figure 3a to Figure 3e in, such as ...

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Abstract

The invention relates to a technical invention which can be used for detecting a protein expression profile of a tissue slice. According to the technical invention, after a plurality of different antibodies are simultaneously bonded with a plurality of proteins to be detected of a tumor tissue, an identification V-D-J sequence subjected to PCR (Polymerase Chain Reaction) amplification is hybridized with an antibody gene chip, and the protein expression profile of the tumor tissue is displayed through a hybridization result; and certain protein in-situ expression statuses are selectively displayed through immunohistochemistry. The technical invention can be used for clinically detecting individual tumor related protein expression profiles of patients, solving the problem that a large number of protein expressions are subjected to in-situ detection for tissue slices, and directly applying a proteome research result to a clinical diagnosis process, thereby being an important premise for realizing individual treatment of diseases.

Description

technical field [0001] The invention relates to a proteome technology and an antibody gene chip technology for clinically detecting patient's individualized tumor-associated protein expression profile, especially a technical invention capable of detecting the protein expression profile of tissue slices. Background technique [0002] The technologies currently used for proteome research mainly include 2D electrophoresis and mass spectrometry, and antibody chips have also been reported for proteome research. These techniques are very expensive, the relevant reagents are not easy to store, and the detection time is long; the detection sensitivity is low, and it is difficult to detect low-abundance proteins. These conditions lead to proteome research techniques and results can not be directly applied to the clinical diagnosis and treatment process. In addition, all current proteome research techniques cannot be directly used for the detection of tissue sections. Contents of t...

Claims

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Application Information

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IPC IPC(8): G01N33/577C12Q1/68
Inventor 朱有凯
Owner 朱有凯
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