Kit for early stage colorectal cancer auxiliary diagnosis and use method and detection system thereof

Abstract

The invention provides a kit for early stage colorectal cancer auxiliary diagnosis and a use method and a detection system thereof. The kit comprises a nucleic acid isolation and purification reagent, a DNA (deoxyribonucleic acid) sulfite conversion reagent, a KRAS (kirsten rat sarcoma viral oncogene homolog) gene mutation detection reagent, a BMP3 (bone morphogenetic protein 3) and NDRG4 (N-myc downsteam regulated gene 4) gene methylation detection reagent and a fecal occult blood detection reagent, wherein the nucleic acid isolation and purification reagent is used for separating and purifying the human DNA in a faeces sample; the DNA sulfite conversion reagent is used for performing sulfite conversion on the purified partial human DNA, and is used for subsequent BMP3 and NDRG4 gene methylation detection. Through detecting two kinds of indexes of DNA and fecal occult blood in the faeces sample in a combined way, the kit provided by the invention is used for the early stage screening of the colorectal cancer. Compared with an FOBT (fecal occult blood test) detection method, the kit provided by the method can realize higher sensitivity on the detection of colorectal cancer, particularly the developing period tumor.

Description

technical field [0001] The invention belongs to the field of biotechnology and diagnosis research, and relates to a kit for auxiliary diagnosis of early colorectal cancer, a use method and a detection system. Background technique [0002] Colorectal cancer, also known as colorectal cancer, occurs in the colon (large intestine) or rectum and usually develops slowly. It is the second most common cancer in urban China, with 6% of asymptomatic people over the age of 40 suffering from early bowel cancer. With the changes in food structure and living habits, as well as the increasingly prominent environmental problems, the incidence of colorectal cancer has increased rapidly. According to the "China Tumor Registration Annual Report", intestinal cancer is currently the second most common cancer in China after lung cancer. Major cancers mainly include colon cancer and rectal cancer. At present, 440,000 newly diagnosed cases of colorectal cancer are newly diagnosed in my country eve...

AI Technical Summary

Problems solved by technology

However, there are many disadvantages in FOBT detection: it detects the accompanying symptoms of tumors—hemorrhage, which may cause missed diagnosis due to suspension of bleeding or no bleeding symptoms in the early stage of tumors, which affects the detection sensitivity of tumors, especially early tumor lesions. Currently, the mainstream FOBT The detection technique is 60-87% sensitive for colorectal cancer and 20-43% for advanced tumors

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