Zinc-copper superoxide dismutase SOD1 expression amount molecular detection method and primer

A superoxide, SOD1 technology, used in the determination/inspection of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc.

Active Publication Date: 2015-12-23
TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] At present, the molecular level detection kit of SOD1 has not yet been found in the ma

Method used

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  • Zinc-copper superoxide dismutase SOD1 expression amount molecular detection method and primer
  • Zinc-copper superoxide dismutase SOD1 expression amount molecular detection method and primer
  • Zinc-copper superoxide dismutase SOD1 expression amount molecular detection method and primer

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Embodiment Construction

[0026] specific implementation

[0027] In order to understand the present invention, the present invention will be further described below in conjunction with embodiment: following embodiment is illustrative, not limiting, can not limit protection scope of the present invention with following embodiment.

[0028] The forward and reverse primers of the fluorescent quantitative PCR primers in this method are designed across genomic introns, which avoids the shortcomings of low accuracy and poor repeatability of the results due to the contamination of genomic DNA remaining in the sample, and improves the specificity and the accuracy of the results. Compared with using NCBIblast and using PRIMER5.0 design software to analyze and optimize primers, all indications were controlled within the optimal range, which greatly reduced the formation of primer dimers. Making the primer length up to 25bp also improves the specificity of amplification to a certain extent. There is no need to...

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Abstract

The invention relates to a zinc-copper superoxide dismutase SOD1 expression amount molecular detection method and a primer used in the method. The forward primer sequence is represented by the sequence 1, and the reverse primer sequence is represented by the sequence 2. According to the provided molecular detection method, a qPCR method is adopted for the first time, the content of SOD1 is detected in the gene level; at the same time, a set of targeting primer sequences and inner reference sequences is provided; even if the RNA extracted from a sample is polluted by DNA, the difference of expression amount of SOD1 gene in different human tissues and cells can be stably, specifically, and precisely detected, moreover, the repeatability is extremely high, and solved are the problems that the commonly-used enzyme activity detection kit can be badly influenced by artificial factors, and the stability and repeatability of the kit are both very bad.

Description

technical field [0001] The invention belongs to the field of biological detection, and relates to an in vitro gene molecular diagnosis method, in particular to a molecular detection method for the expression level of zinc-copper superoxide dismutase SOD1 and the primers used. technical background [0002] Superoxide dismutase (Superoxide Dismutase referred to as SOD) is a new enzyme preparation. It is widely distributed in the biological world, almost from animals to plants, and even from humans to single-celled organisms. SOD is regarded as the most magical enzyme in life science and technology. It is the garbage scavenger in the human body. SOD is the natural enemy of oxygen free radicals, the number one killer of oxygen free radicals in the body, and the foundation of life and health. [0003] Due to the pressure of modern life, environmental pollution, various radiation and excessive exercise, etc. will cause the formation of a large number of oxygen free radicals, it h...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6851C12Q1/6883C12Q2600/158C12Q2531/113C12Q2545/101
Inventor 孙麟马洁刘宇
Owner TIANJIN KANGTING BIOLOGICAL ENG GRP CO LTD
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