Use of agarose in DNA extraction, DNA extraction kit and DNA extraction method

An extraction method and agarose technology, applied in the direction of DNA preparation, recombinant DNA technology, etc., can solve the problems of low DNA detection rate, failure of STR typing, failure to identify criminal individuals, etc., achieve sufficient response, increase recovery rate, complete The effect of STR typing results

Inactive Publication Date: 2016-06-29
武汉瑞博祥生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the little DNA residue at the crime scene, the forensic evidence DNA recovered by the above-mentioned conventional methods is extremely small, which directly leads to the low detection rate of subsequent DNA, the failure of STR typing, and the inability to identify criminal individuals

Method used

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  • Use of agarose in DNA extraction, DNA extraction kit and DNA extraction method
  • Use of agarose in DNA extraction, DNA extraction kit and DNA extraction method
  • Use of agarose in DNA extraction, DNA extraction kit and DNA extraction method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Application of agarose for DNA extraction from trace bloodstains.

[0033] Weigh 0.05 g of agarose and add it to 100 g of water, mix evenly to dissolve it, and obtain the agarose fluid for use.

[0034] A DNA extraction kit includes a box body and reagents stored separately in the box body, and the reagents include: agarose, dithiothreitol, proteinase K, and Chelex-100.

[0035] The agarose is an agarose fluid obtained by dissolving 0.05 g of agarose in 100 g of water.

[0036] The concentrations of the other components are respectively 1 M of dithiothreitol, 5 mg / ml of protease K, and 5% of Chelex-100.

[0037] The above DNA extraction kit was used for DNA extraction from trace bloodstains.

[0038] Set up experimental group 1 and experimental group 2, and use two different methods to extract DNA from the same blood card. Experimental group 1 uses a DNA extraction kit for DNA extraction from trace blood stains, and experimental group 2 uses the conventional Chelex-10...

Embodiment 2

[0047] Agarose was applied to the extraction of DNA from the trace amount of oral exfoliated cells remaining on cigarette butts.

[0048] Weigh 0.2 g of agarose and add it to 100 g of water, mix evenly to dissolve it, and obtain the agarose fluid for use.

[0049] A DNA extraction kit includes a box body and reagents stored separately in the box body, and the reagents include: agarose, dithiothreitol, proteinase K, and Chelex-100.

[0050] The agarose is an agarose fluid obtained by dissolving 0.2 g of agarose in 100 g of water.

[0051] The concentrations of the other components are 0.5 M of dithiothreitol, 3.5 mg / ml of proteinase K, and 8% of Chelex-1008, respectively.

[0052] The above-mentioned DNA extraction kit was used for the extraction of DNA from a small amount of oral exfoliated cells remaining on cigarette butts.

[0053] The cigarette butts provided by the volunteers were collected, and the trace DNA of oral exfoliated cells remaining on the same cigarette butt...

Embodiment 3

[0065] Agarose was applied to the extraction of DNA from nail exfoliated cells.

[0066] Weigh 0.4 g of agarose and add it into 100 g of water, mix evenly to dissolve it, and obtain the agarose fluid after complete dissolution.

[0067] A DNA extraction kit includes a box body and reagents stored separately in the box body, and the reagents include: agarose, dithiothreitol, proteinase K, and Chelex-100.

[0068] The agarose is an agarose fluid obtained by dissolving 0.4 g of agarose in 100 g of water.

[0069] The concentrations of the other components are 1.8 M of dithiothreitol, 6.0 mg / ml of proteinase K, and 6% of Chelex-1000, respectively.

[0070] The above DNA extraction kit was used for DNA extraction of trace nail exfoliated cells.

[0071] The nails provided by the volunteers were collected, and DNA from nail exfoliated cells of the same nail sample was extracted by two different methods.

[0072] Experimental group 6 and experimental group 7 were set up. Experimen...

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Abstract

The invention provides a use of agarose in DNA extraction. In DNA extraction, agarose is used. The invention further provides a DNA extraction kit and a DNA extraction method. The DNA extraction kit comprises a box and an individually stored reagent in the box. The reagent comprises agarose. The DNA extraction method comprises adding agarose in DNA extraction. An experiment proves that through use of agarose in DNA extraction, a recovery rate of a trace amount of DNA in extraction is improved and good PCR amplification effects and a complete STR parting result are obtained. Compared with the conventional extraction methods such as a Chelex-100 method and a paramagnetic particle method, the DNA extraction method provided by the invention has a higher DNA recovery rate.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of agarose in DNA extraction, a DNA extraction kit and a DNA extraction method. Background technique [0002] For extremely small amounts of DNA samples, it is difficult for conventional PCR amplification systems to capture effective DNA information. The low recovery rate of the conventional DNA extraction method for the extraction of trace DNA samples is the main reason for the poor effect of subsequent PCR amplification. For example, in the work of forensic investigation, the identification and detection of forensic evidence at the crime scene plays a vital role in timely and effectively identifying criminals and combating crimes. Bloodstains, cigarette butts, fingerprints, etc. at the crime scene all contain trace amounts of DNA information, which can provide scientific basis for identifying or proving criminal suspects. [0003] At present, the techn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10
Inventor 罗冰科佟浩朱传红徐婷
Owner 武汉瑞博祥生物科技有限公司
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