1 results about "Heat inactivation" patented technology

The application relates to a glutamine transaminase with improved thermal stability and application thereof, and belongs to the technical field of genetic engineering and protein engineering. The variant comprises a leading peptide and a mature polypeptide. The parent mature polypeptide of the variant corresponds to the region of the N terminal 4th to 13th of the wild-type SmTG mature polypeptide, and is replaced by a target sequence composed of n repeating units connected head to tail. The repeating unit has at least 80% identity with the original sequence, and is independently substituted by a specific amino acid at the corresponding 10th position, wherein at least two repeating units have different types of substitution amino acids at the 10th position. The remaining amino acid region of the parent mature polypeptide has at least 85% sequence identity with the remaining region of the wild-type SmTG mature polypeptide. Compared with the parent, the variant has a glutamine transaminase activity, and the thermal inactivation half-life at 60 DEG C is significantly prolonged by at least 0.3 times.