Inactivated lactobacillus effervescent tablets and preparation method thereof

A technology of inactivating lactic acid bacteria and inactivating lactic acid bacteria powder, which is applied in the field of health products and beverages, can solve the problems of difficult control of hygienic indicators, large consumption of cans, packaging bottles, and post-fermentation of products, and achieves simple and feasible preparation methods, extended shelf life, non-perishable effect

Active Publication Date: 2013-07-24
胡文锋
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The advantage of liquid beverages is that they can be drunk directly after opening, but the production and use of liquid beverages will consume a lot of cans and packaging bottles, and cause certain pollution to the environment. Carry it with you, especi

Method used

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  • Inactivated lactobacillus effervescent tablets and preparation method thereof
  • Inactivated lactobacillus effervescent tablets and preparation method thereof
  • Inactivated lactobacillus effervescent tablets and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0052] The preparation of embodiment 1 inactivated lactic acid bacteria powder

[0053] S01. Inoculate Lactobacillus acidophilus (purchased from China Industrial Microorganism Culture Collection and Management Center, and those skilled in the art can also use related bacteria from other sources) according to the inoculum size of 5-10% in the conventional MRS liquid medium Cultivate at 37°C, when the number of lactic acid bacteria reaches 10 9 / mL, the culture was stopped to obtain the fermentation broth;

[0054] S02. Add glycerin or sodium alginate with a mass fraction of 0.1 to 0.5% to the fermentation broth obtained in S01 to obtain a mixed system;

[0055] S03. Heat inactivate the mixed system obtained from S02 at 60°C for 30 minutes, centrifuge at 3000-5000rmp for 5-10 minutes to collect lactic acid bacteria cells;

[0056] S04. Add soluble starch of equal quality to the thallus obtained in S03, carry out vacuum drying (60°C, 0.04MPa) to prepare inactivated lactic acid ...

Embodiment 2

[0057] The preparation of embodiment 2 inactivated lactic acid bacteria powder

[0058] S01. Inoculate Lactobacillus bulgaricus and Streptococcus thermophilus (purchased from China Industrial Microbiology Culture Collection Management Center) into milk liquid culture medium at 37°C according to the inoculation amount of 5-10%, and cultivate them when the number of lactic acid bacteria reaches 10 8-9 / mL, the culture was stopped to obtain a fermented liquid; the composition of the milk liquid medium was: 8% skimmed milk powder, 2% sucrose, and 90% water in terms of mass percentage.

[0059] S02. Add glycerin or sodium alginate with a mass fraction of 0.1 to 0.5% to the fermentation broth obtained in S01 to obtain a mixed system;

[0060] S03. Heat inactivate the mixed system obtained from S02 at 80°C for 20 minutes, centrifuge at 3000-5000rmp for 5-10 minutes to collect lactic acid bacteria cells;

[0061] S04. Add glucose of equal quality to the thallus obtained in S03, carry...

Embodiment 3

[0062] The preparation of embodiment 3 inactivated lactic acid bacteria powder

[0063] S01. Inoculate Bifidobacteria (purchased from China Industrial Microorganism Culture Collection Management Center) into conventional PYG liquid medium according to 5-10% inoculum size for anaerobic culture at 37°C. When the number of lactic acid bacteria reaches 10 7-8 / mL, the culture was stopped to obtain the fermentation broth;

[0064] S02. Adding glycerol and sodium alginate with a mass fraction of 0.1 to 0.5% in the fermentation broth obtained in S01 to obtain a mixed system;

[0065] S03. heat-inactivate the mixed system obtained in S02 at 60-80°C for 30-60 minutes, and centrifuge at 3000-5000rmp to collect lactic acid bacteria cells;

[0066] S04. Add glucose of equal quality to the thalline obtained in S03, spray dry (inlet temperature 150°C, outlet temperature 105°C) to prepare inactivated lactic acid bacteria powder, and the number of bacteria in the powder reaches 10 9 piece / g...

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Abstract

The invention discloses effervescent tablets with inactivated lactobacillus cells as an effective component, and a preparation method thereof. The inactivated lactobacillus effervescent tablets are prepared from the raw materials of, by weight, 5-15% of inactivated lactobacillus powder, 20-30% of oligosaccharide, 23-33% of an acid source, 25-35% of an alkali source, 2-5% of a disintegrant, and 2-5% of a lubricant. According to the invention, the lactobacillus cells are inactivated through a heat inactivation method. The effervescent tablets comprise lactobacillus cells, and have the functions of digestion promoting, intestinal micro-ecological balance maintaining, and human immunity improving. Also, the tablets comprise oligosaccharide bifidus factors, and can promote probiotic growth in bodies, such that a dual health-care effect is achieved. The effervescent tablets are convenient to carry and to eat. When the effervescent tablet is dissolved in proper amount of drinking water, an instant beverage can be obtained. The tablets are solid health-care beverages for various populations. The effervescent tablet preparation method provided by the invention is simple and feasible, and has important actual application and popularization values.

Description

technical field [0001] The invention relates to the technical field of health food beverages, and more specifically relates to an inactivated lactic acid bacteria effervescent tablet and a preparation method thereof. technical background [0002] Lactic acid bacteria are a common type of probiotics in the human intestinal tract. It can improve the micro-ecological balance in the human intestinal tract, inhibit the growth of Escherichia coli, Staphylococcus aureus, Salmonella and other pathogenic bacteria, promote digestion, and improve human immunity. In recent years, its various health functions have been confirmed by experts and scholars at home and abroad, and the products developed with it are also dazzling, such as yogurt, lactic acid bacteria drinks, cheese, etc. Among them, the active liquid lactic acid bacteria drink is particularly prominent. The advantage of liquid beverages is that they can be drunk directly after opening, but the production and use of liquid bev...

Claims

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Application Information

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IPC IPC(8): A23L1/09A23P1/02
Inventor 胡文锋庞旭韩笑刘秉杰杨益衡
Owner 胡文锋
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