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Preparation method of whole cell suspension of streptococcus pyogenes and serratia marcescens

A technology of Serratia marcescens and Streptococcus pyogenes, which is applied in the field of preparation of whole cell suspensions of Streptococcus pyogenes and Serratia marcescens, can solve the problem that the anti-tumor mechanism is not completely clear and the curative effect is not good , No reports of anti-tumor activity etc.

Inactive Publication Date: 2011-11-09
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antitumor drugs made of bacterial components such as ∝-mannan peptides, lipoteichoic acid, DNA, etc. have definite curative effects and are easy to control. Component mixtures (bacterial DNA, bacterial metabolites, etc.) are not more effective in treating tumors than existing anti-tumor drugs such as paclitaxel
[0019] Existing research materials and technical literature show that the whole-cell preparations of various strains of Streptococcus pyogenes have anti-tumor pharmacological activity, but there is no anti-tumor activity report on the whole-cell preparations of other strains of Serratia marcescens

Method used

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  • Preparation method of whole cell suspension of streptococcus pyogenes and serratia marcescens
  • Preparation method of whole cell suspension of streptococcus pyogenes and serratia marcescens
  • Preparation method of whole cell suspension of streptococcus pyogenes and serratia marcescens

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Embodiment 1A

[0033] The preparation of embodiment 1ATCC43820 and ATCC21059 composite bacterial whole cell suspension

[0034] Strains: Streptococcus pyogenes ATCC21059 strain, Serratia marcescens ATCC43820 strain

[0035] Streptococcus pyogenes ATCC21059 strain and Serratia marcescens ATCC43820 strain were cultured separately and mixed in proportion.

[0036] 1. Strain passage

[0037] Medium composition: Each liter of double distilled water contains 3.0 g of beef extract, 10.0 g of Neopeptone (Neopeptone), and 5.0 g of sodium chloride.

[0038] Culture conditions: pH 7.5; 50rpm shaking culture, amplitude 19mm, Serratia marcescens strains were cultured at 25°C; strains of Streptococcus pyogenes were cultured at 37°C.

[0039] Number of passages: the number of passages after the opening of the main seed batch is 1 generation; the number of passages from the opening of the working seed batch to the inoculation of the production medium is 1 generation.

[0040] 2. Preparation of Composite ...

Embodiment 2A

[0044] The preparation of embodiment 2ATTCC43821 and ATCC21060 composite bacterial whole cell suspension

[0045] Strains: Streptococcus pyogenes ATCC21060 strain, Serratia marcescens ATTCC43821 strain

[0046] 1. Strain passage

[0047] Medium composition: Each liter of double distilled water contains 3.0 g of beef extract, 10.0 g of Neopeptone (Neopeptone), and 5.0 g of sodium chloride.

[0048] Culture conditions: pH 7.0; 50rpm shaking culture, amplitude 19mm, Serratia marcescens cultured at 27°C; strain Streptococcus pyogenes cultured at 37°C.

[0049] Number of passages: The number of passages after the main seed batch is opened is 2 generations; the number of passages from the opening of the working seed batch to the inoculation of the production medium is 1 generation.

[0050] 2. Preparation of Composite Bacterial Whole Cell Suspension

[0051] Medium: Each liter of double distilled water contains 3.0 g of beef extract, 10.0 g of Neopeptone, and 5.0 g of sodium chlo...

Embodiment 3A

[0054] The preparation of embodiment 3ATCC13880 and ATCC700924 composite bacterial whole cell suspension

[0055] Strains: Streptococcus pyogenes ATCC700924 strain, Serratia marcescens ATCC13880 strain

[0056] 1. Strain passage

[0057] Medium composition: Each liter of double distilled water contains 3.0 g of beef extract, 10.0 g of Neopeptone (Neopeptone), and 5.0 g of sodium chloride.

[0058] Culture conditions: pH 7.3; 50rpm shaking culture, amplitude 19mm, Serratia marcescens cultured at 25°C; strain Streptococcus pyogenes cultured at 37°C.

[0059] Number of passages: The number of passages after the opening of the main seed batch is 2 generations; the number of passages from the opening of the working seed batch to the inoculation of the production medium is 2 generations.

[0060] 2. Preparation of Composite Bacterial Whole Cell Suspension

[0061] Medium: Each liter of double distilled water contains 3.0 g of beef extract, 10.0 g of Neopeptone, and 5.0 g of sodiu...

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Abstract

The invention discloses a preparation method of a whole cell suspension of streptococcus pyogenes and serratia marcesens, comprising the following steps of: respectively culturing streptococcus pyogenes and serratia marcesens to obtain whole cell culture solutions, then mixing the two bacteria according to the ratio of 1:1 or 2:1 as per the bacterial number, and after heat inactivation, diluting by a phenol normal saline of 1-3g / l containing phenol or resorcinol to obtain the whole cell suspension. In the whole cell suspension of streptococcus pyogenes and serratia marcesens prepared by the invention, the total number of bacteria of the streptococcus pyogenes and the serratia marcesens is 100000-3000000 per milliliter, and the whole cell suspension of streptococcus pyogenes and serratia marcesens can be used for treating tumor by the routes of administration such as intratumor injection, intracavitary injection or intramuscular injection.

Description

technical field [0001] The invention relates to a preparation method of a medicine with antitumor effect, in particular to a preparation method of the whole cell suspension of Streptococcus pyogenes and Serratia marcescens. Background technique [0002] In recent years, there are 1.6-1.7 million new cancer patients in my country every year, and the total number is about 4.5 million. Both its morbidity and mortality are on the rise. According to the "Summary of China's Health Statistics in 2009" released by the Ministry of Health, among the top ten disease-related death rates in some cities and counties in 2008, malignant tumors ranked first, and they have become the number one killer that endangers human health. [0003] There are many anti-tumor chemotherapeutic drugs in the prior art, and more compounds have been found to have anti-tumor effects. However, due to the lack of ideal selectivity, most anti-tumor drugs have inhibitory or damaging effects on normal human tissu...

Claims

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Application Information

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IPC IPC(8): A61K35/74A61K9/10A61P35/00A61K35/742
Inventor 厉保秋
Owner SHANDONG UNIV
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