Graphene and protein composite film based on non-covalent modification and preparation method thereof

Abstract

The invention relates to a graphene and protein composite film based on non-covalent modification and a preparation method thereof. The composite film comprises a silica substrate, single-layer graphene and a protein film. The preparation method comprises the following steps: transferring the single-layer graphene film grown through chemical vapor deposition onto the surface of the silica substrate, removing a photoresist on the surface of the graphene film to leave single-layer graphene on the surface of the silica substrate; and dropwise adding the a protein solution onto the surface of the single-layer graphene and carrying out heat inactivation treatment at 60-90 DEG C for 1-10 min to form a protein film on the surface of the single-layer graphene. Non-covalent modification of the protein film is realized on the surface of graphene through the heat inactivation method. Thickness of the graphene-protein film can be controlled to nanoscale. The film is uniform. The preparation method is simple to operate and easy to realize. The protein film surface has amino group and carboxyl group and can be used as a platform for follow-up biomolecule diagnosis. The product has a good application prospect.

Description

technical field [0001] The invention belongs to the field of graphene biosensors, in particular to a graphene protein composite film based on non-covalent modification and a preparation method. Background technique [0002] Graphene is a two-dimensional material with a single-layer carbon atomic structure. Due to its stable chemical structure and excellent optical, mechanical and electrical properties, graphene is an ideal material in the field of nano-bioelectric detection. Graphene devices are used to detect proteins, DNA, nerve cell action potentials, and bacteria. In the construction process of graphene biosensors, the biomolecular modification of the graphene surface is a crucial link. The modification methods of graphene surface can generally be divided into covalent modification and non-covalent modification. Covalent modification of graphene is usually stable but will damage the electrical properties of graphene, while non-covalent modification can usually effectiv...

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Problems solved by technology

The coupling molecules of 1-pyrenebutanoic acid succinimidyl ester and NHSester tripod are sensitive to light and humidity, and the adsorption amount of peptide chains and BSA on the surface of graphene is small, and the adsorption amount of coupling molecules is usually uncontrollable, which is difficult to meet the needs of graphene applications. Practical needs in biosensors

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