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Method for preparing enzyme electrode with MWCNTs-TiO2/Nafion composite medium

A composite medium and enzyme electrode technology, applied in the field of electrochemistry, can solve problems such as poor conductivity

Inactive Publication Date: 2009-12-16
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Recently it was found that protein molecules and TiO 2 There is a strong electrostatic adsorption between them, and through this electrostatic interaction, the protein can be adsorbed on TiO 2 surface is immobilized, but the TiO 2 The conductivity itself is not very good, and TiO needs to be 2 modify or modify

Method used

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  • Method for preparing enzyme electrode with MWCNTs-TiO2/Nafion composite medium
  • Method for preparing enzyme electrode with MWCNTs-TiO2/Nafion composite medium
  • Method for preparing enzyme electrode with MWCNTs-TiO2/Nafion composite medium

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Embodiment 1

[0051] 1. MWCNTs-TiO 2 The preparation steps of the enzyme electrode of / Nafion composite medium are as follows:

[0052] (1) MWCNTs-TiO 2 Preparation of core-shell nanocomposites

[0053] MWCNTs multi-walled carbon nanotubes were reflux oxidized in concentrated nitric acid at 140 °C for 10 h. The acid-treated MWCNTs were dispersed in 1% sodium dodecyl sulfate solution, ultrasonicated for 3 hours to make sodium dodecyl sulfate adsorb on the surface of the MWCNTs, rinsed repeatedly, and dried. Add 100 μL of n-butyl titanate, 100 μL of water, and 5 mg of MWCNTs adsorbed with sodium dodecyl sulfate to 4 mL of absolute ethanol. After sonicating for 30 min, the suspension was transferred to beaker A (volume 10 mL). Inject 4mL of double distilled water into beaker B (volume 20mL), then place beaker A in beaker B, seal the device, place it in an oven and heat at 80°C for 3h. After the reaction, the centrifuged solid sample was repeatedly washed with absolute ethanol and double d...

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Abstract

The invention discloses a method for preparing an enzyme electrode with an MWCNTs-TiO2 / Nafion composite medium. In the method, MWCNTs-TiO2 core-shell nanocomposite materials are scattered into Nafion to prepare an organic-inorganic composite membrane as a fixed matrix for biological molecules for structuring a hemoglobin electrode, and a hemoglobin biosensor with fast response, high sensitivity and strong catalytic capacity is prepared by using the properties of large specific surface area, high surface reactivity, strong adsorption capacity, large electrical conductivity and the like of the MWCNTs-TiO2 core-shell nanocomposite materials and the properties of membrane forming, high chemical stability, high anti-interference capacity and the like of the Nafion. The biosensor has good biocompatibility, stability and repeatability, and has potential application in structuring biosensors. The sensor can be used for detecting the substances of hydrogen peroxide, trichloroacetic acid and the like, and has the advantages of low sensitivity, low detection limit and the like.

Description

technical field [0001] The invention belongs to the field of electrochemistry, and in particular relates to a method for preparing an enzyme electrode for measuring certain substances in a sample by using an electrochemical method. Background technique [0002] At present, more and more scholars are committed to the direct electrochemical research of redox proteins, because these research works can not only help us understand the structure of proteins, protein molecular redox conversion mechanism and metabolic process, but also promote amperometric biosensors and Further development of bioelectrocatalytic systems. Hemoglobin is a type of heme protein that transports oxygen and carbon dioxide molecules in red blood cells. Hemoglobin is a tetrameric oligomeric protein composed of two α- and two β-polypeptide chains. Each chain is bound to a heme group. The helical structure of each polypeptide chain forms a hydrophobic space. Can protect the heme molecule from contact with w...

Claims

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Application Information

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IPC IPC(8): G01N27/327G01N27/48
Inventor 田丹碧马伟杨孝荣徐娟
Owner NANJING UNIV OF TECH
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