Preparation method of graphene immobilized laccase
A technology of graphene and laccase, which is applied to biochemical equipment and methods, and enzymes immobilized on or in inorganic carriers, can solve the problems of expensive synthesis, complicated processing technology, and poor separation effect, and achieve enzyme retention Live, improve stability, avoid chemical damage
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[0025] Example one
[0026] Carrier preparation, its steps are:
[0027] (1) Weigh accurately 5g of dodecylamine powder, dissolve it in 20ml of absolute ethanol, weigh 1.590g of ferrous chloride tetrahydrate and 0.900g of ammonium dihydrogen phosphate and dissolve in 80ml of ultrapure water (dihydrogen phosphate The molar ratio of ammonium to ferrous chloride tetrahydrate is 1:1.02, and the molar ratio of ammonium dihydrogen phosphate to dodecylamine is 1:3.45). In a 45°C water bath, mix the dodecylamine absolute ethanol solution with the mixed solution of ferrous chloride and ammonium dihydrogen phosphate, centrifuge to collect the solid product, and vacuum dry at 40°C for 36 hours. The dried solid product was ground into powder and placed in a crucible. After drying, put the crucible into a tube furnace and calcinate at 600°C for 24h under a low flow argon atmosphere;
[0028] (2) After the calcination process, after the product is cooled to room temperature, scrape the product ...
Example Embodiment
[0032] Example two
[0033] Carrier preparation, its steps are:
[0034] (1) Weigh accurately 5g of dodecylamine powder, dissolve it in 20ml of absolute ethanol, weigh 1.590g of ferrous chloride tetrahydrate and 0.900g of ammonium dihydrogen phosphate and dissolve in 80ml of ultrapure water (dihydrogen phosphate The molar ratio of ammonium to ferrous chloride tetrahydrate is 1:1.02, and the molar ratio of ammonium dihydrogen phosphate to dodecylamine is 1:3.45). In a 45°C water bath, mix the dodecylamine absolute ethanol solution with the mixed solution of ferrous chloride and ammonium dihydrogen phosphate, centrifuge to collect the solid product, and vacuum dry at 40°C for 36 hours. The dried solid product was ground into powder and placed in a crucible. After drying, put the crucible into a tube furnace and calcinate at 600°C for 24h under a low flow argon atmosphere;
[0035] (2) After the calcination process, after the product is cooled to room temperature, scrape the product ...
Example Embodiment
[0039] Example three
[0040] Carrier preparation, its steps are:
[0041] (1) Weigh accurately 5g of dodecylamine powder, dissolve it in 20ml of absolute ethanol, weigh 1.590g of ferrous chloride tetrahydrate and 0.900g of ammonium dihydrogen phosphate and dissolve in 80ml of ultrapure water (dihydrogen phosphate The molar ratio of ammonium to ferrous chloride tetrahydrate is 1:1.02, and the molar ratio of ammonium dihydrogen phosphate to dodecylamine is 1:3.45). In a 45°C water bath, mix the dodecylamine absolute ethanol solution with the mixed solution of ferrous chloride and ammonium dihydrogen phosphate, centrifuge to collect the solid product, and vacuum dry at 40°C for 36 hours. The dried solid product was ground into powder and placed in a crucible. After drying, put the crucible into a tube furnace and calcinate at 600°C for 24h under a low flow argon atmosphere;
[0042] (2) After the calcination process, after the product is cooled to room temperature, scrape the produc...
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