Human platelet antigen genotyping liquid chip and human platelet antigen genotyping detection method thereof

A human platelet and antigen gene technology, applied in the field of molecular biology, can solve the problems of poor accuracy, sensitivity, and low throughput, and achieve the effect of simple operation, high sensitivity, and stable results

Inactive Publication Date: 2013-01-09
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

[0005] The purpose of the present invention is to provide a human platelet antigen genotyping liquid chip with higher accuracy, sensitivity and flexibility and its detection method, which can detect 22 kinds of human platelet antigen types officially named at one time, To overcome the problems of low throughput, poor accuracy and sensitivity in the current human platelet antigen genotyping technology

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  • Human platelet antigen genotyping liquid chip and human platelet antigen genotyping detection method thereof
  • Human platelet antigen genotyping liquid chip and human platelet antigen genotyping detection method thereof
  • Human platelet antigen genotyping liquid chip and human platelet antigen genotyping detection method thereof

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Embodiment Construction

[0035] The present invention will be described in detail below in combination with specific embodiments.

[0036]1. Liquid chip (liquichip), also known as suspension array technology (suspension array technology, SAT), is a new generation of biochip technology. The liquid phase chip system is mainly composed of four elements: microspheres, capture molecules (antigens), antibodies or nucleic acid probes, molecules to be tested, and reporter molecules. The microspheres are made of polystyrene and are mainly used to immobilize and distinguish different capture molecules; using fluorescent coding technology, that is, two red fluorescent dyes with different ratios are precisely mixed in the microsphere preparation process, and 100 capture molecules can be obtained. A fluorescently encoded microsphere with different colors can be used to label 100 different probe molecules, and it can also detect up to 100 different molecules to be detected in a sample at the same time. If the capt...

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Abstract

The invention belongs to the technical field of molecular biology, and in particular relates to a human platelet antigen genotyping liquid phase chip and a detection method thereof. Human platelet antigen genotyping technology is an important technical means to provide patients with matching platelets and promote the safety and effectiveness of clinical platelet transfusion. However, the existing human platelet antigen genotyping technology has low throughput and high accuracy. Indexes such as sensitivity and sensitivity need to be improved. The liquid phase chip of the present invention includes primers 1-24, connection probes 25-46 and fluorescently encoded microspheres coated with detection probes 47-68, and the human platelet antigen gene is completed through amplification, connection and hybridization reactions. Typing detection. The liquid phase chip and detection method adopted in the present invention have the advantages of large flux, high sensitivity, strong specificity, good repeatability, wide linear range, simple operation, strong flexibility, and wide application range, and are clinically accurate, Efficient and practical human platelet antigen genotyping detection method.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a human platelet antigen genotyping liquid phase chip and a detection method thereof. Background technique [0002] Platelet transfusion is an important means to prevent and treat bleeding disorders caused by thrombocytopenia or abnormal function. Due to the limitations of the current human platelet antigen (HPA) typing technology, ABO blood type is often considered during clinical platelet transfusion, while the matching of platelet blood type is ignored. Platelet blood group incompatibility can lead to a series of clinical alloimmune symptoms, such as neonatal alloimmune thrombocytopenia (NAIT), post-transfusion purpura (PTP) and platelet refractory transfusion (PTR), etc. Establishing a new, efficient and practical HPA typing technology to provide patients with matching platelets is undoubtedly of great significance for promoting the safety and effective...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N21/64
Inventor 安群星李翠莹白艳军穆士杰
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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