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Application of chloride salt in the preparation of biochemical reagents that eliminate negative or zero values ​​in assay results

A biochemical reagent, chloride salt technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve the problem of inability to accurately determine the concentration of chemical components in patients, and achieve the effect of eliminating zero or negative values.

Active Publication Date: 2016-02-17
SICHUAN XINCHENG BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current biochemical reagents cannot accurately measure the concentration of these chemical components in the body of some patients

Method used

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  • Application of chloride salt in the preparation of biochemical reagents that eliminate negative or zero values ​​in assay results
  • Application of chloride salt in the preparation of biochemical reagents that eliminate negative or zero values ​​in assay results
  • Application of chloride salt in the preparation of biochemical reagents that eliminate negative or zero values ​​in assay results

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Application of chloride salt in the preparation of biochemical reagents that eliminate negative or zero values ​​in assay results. Adding chloride salts to biochemical reagents, chloride salts can provide a suitable storage and reaction environment for the analyte, so that the analyte exhibits due biological activity and maintains the natural conformation of the analyte; and participates in The composition of the substance to be tested or the active center of the tool enzyme ensures the normal progress of the reaction and obtains real test results, and eliminates the phenomenon of zero or negative values ​​in the test sample results.

Embodiment 2

[0023] The application of chloride salt in the preparation of biochemical reagents that eliminate negative or zero values ​​in the determination results, adding chloride salts during the configuration of biochemical reagents. The chloride salt is NaCl or MgCl 2 or CaCl 2 . NaCl, MgCl 2 , CaCl 2 The concentrations of the three are 1-50g / L. Biochemical reagents include biochemical reagents for creatinine determination, biochemical reagents for total bile acid determination and biochemical reagent configuration for adenosine deaminase determination. NaCl, MgCl 2 , CaCl 2 The three salts usually exist in the human body or animal body. The selection of these three salts can provide a suitable storage and reaction environment for the analyte, so that the analyte can show its due biological activity. It maintains the natural structure of the analyte and participates in the formation of the analyte or tool enzyme activity center, which ensures the normal progress of the reactio...

Embodiment 3

[0025] The concentration of creatinine in the patient's serum is measured with a biochemical reagent for creatinine determination. The biochemical reagent is a double reagent. The specific process is:

[0026] The process of configuring the R1 reagent biochemical reagent is as follows: add about 700ml of purified water to a 1000ml clean beaker (the quality of the purified water meets the water requirements for in vitro diagnostic reagents), and then weigh 50mmol / L of HEPES, that is, 2-[4-(2-hydroxyethyl base)-1-piperazine]ethanesulfonic acid 11.92g, add to the beaker and stir to dissolve, then add 1ml of surfactant TX-100, namely Triton-100, stir to dissolve, then add TOOS, namely N-ethyl-N -(2-Hydroxy-3-sulfopropyl)-3-methylaniline 0.99g, add 1-10g NaCl, stir to dissolve and mix well, use 20% NaOH to initially adjust pH to 7.6, dilute to 1000ml, then add Sarcosine oxidase 10ku, creatine amidinohydrolase 50ku;

[0027] The process of configuring the R2 reagent biochemical re...

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Abstract

The invention discloses an application of chlorate in preparation of a biochemical reagent for eliminating a negative value or a zero value in a determination result. According to a novel use of the chlorate, the chlorate capable of providing a proper preservation and reaction environment to a to-be-determined object is added in the biochemical reagent, so that the to-be-determined object presents deserved biological activities, and the native conformation of the to-be-determined object is maintained; and the constitution of the to-be-determined object or a tool enzyme active center is participated, the normal operation of a reaction is guaranteed, a real detection result is obtained, and a phenomenon of the negative value or the zero value in the sample detection result is eliminated.

Description

technical field [0001] The invention belongs to the field of biological detection, and in particular relates to the application of chloride salt in the preparation of biochemical reagents for eliminating negative or zero values ​​in measurement results. Background technique [0002] The instruments currently used to detect biochemical indicators in the body mainly include high-performance liquid chromatography and automatic biochemical detection instruments. Detection by high performance liquid chromatography is a recognized detection method with accurate detection results. However, due to the cumbersome operation of this method, long sample processing time and the need to use relatively expensive instruments for support. With the development and improvement of modern enzymology and automatic biochemical analyzers, more and more disease markers can be detected by designing biochemical reagents. The automatic biochemical analyzer is an instrument for measuring a specific che...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/34C12Q1/32C12Q1/26C12Q1/527C12Q1/28C12Q1/44
Inventor 蒲建文吴明香
Owner SICHUAN XINCHENG BIOLOGICAL CO LTD
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