Human platelet antigen genotyping liquid chip and human platelet antigen genotyping detection method thereof

A human platelet and antigen gene technology, applied in the field of molecular biology, can solve the problems of low throughput, poor accuracy and sensitivity, and achieve high sensitivity, easy operation and stable results

Inactive Publication Date: 2011-11-02
FOURTH MILITARY MEDICAL UNIVERSITY
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Problems solved by technology

[0005] The purpose of the present invention is to provide a human platelet antigen genotyping liquid chip with higher accuracy, sensitivity and flexibility and its detection method, which can detect 22

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  • Human platelet antigen genotyping liquid chip and human platelet antigen genotyping detection method thereof
  • Human platelet antigen genotyping liquid chip and human platelet antigen genotyping detection method thereof
  • Human platelet antigen genotyping liquid chip and human platelet antigen genotyping detection method thereof

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Embodiment Construction

[0035] The present invention will be described in detail below in combination with specific embodiments.

[0036]1. Liquid chip (liquichip), also known as suspension array technology (suspension array technology, SAT), is a new generation of biochip technology. The liquid phase chip system is mainly composed of four elements: microspheres, capture molecules (antigens), antibodies or nucleic acid probes, molecules to be tested, and reporter molecules. The microspheres are made of polystyrene and are mainly used to immobilize and distinguish different capture molecules; using fluorescent coding technology, that is, two red fluorescent dyes with different ratios are precisely mixed in the microsphere preparation process, and 100 capture molecules can be obtained. A fluorescently encoded microsphere with different colors can be used to label 100 different probe molecules, and it can also detect up to 100 different molecules to be detected in a sample at the same time. If the capt...

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Abstract

The invention belongs to the technical field of molecular biology and particularly relates to a human platelet antigen genotyping liquid chip and a human platelet antigen genotyping detection method. A human platelet antigen genotyping technique is an important safe and efficient technical means of providing matching platelets for patients and promoting clinic platelet injection, but the conventional human platelet antigen genotyping technique has the problems of low flux, low accuracy, low sensitivity and other low indexes and the like. The liquid chip provided by the invention contains primers numbered from 1 to 24, connecting probes numbered from 25 to 46 and fluorescent coded microspheres coated with detection probes numbered from 47 to 68 and can accomplish human platelet antigen genotyping detection by amplification, chain connection and crossing reaction. The liquid chip and the detection method, which are adopted by the invention, have the advantages of high flux, high sensitivity, high specificity, high repeatability, wide linear range, simple operation, high flexibility and wide application range, and the method is an accurate, efficient and practical human platelet antigen genotyping detection method for use in clinic.

Description

technical field [0001] The invention belongs to the technical field of molecular biology, and in particular relates to a human platelet antigen genotyping liquid phase chip and a detection method thereof. Background technique [0002] Platelet transfusion is an important means to prevent and treat bleeding disorders caused by thrombocytopenia or abnormal function. Due to the limitations of the current human platelet antigen (HPA) typing technology, ABO blood type is often considered during clinical platelet transfusion, while the matching of platelet blood type is ignored. Platelet blood group incompatibility can lead to a series of clinical alloimmune symptoms, such as neonatal alloimmune thrombocytopenia (NAIT), post-transfusion purpura (PTP) and platelet refractory transfusion (PTR), etc. Establishing a new, efficient and practical HPA typing technology to provide patients with matching platelets is undoubtedly of great significance for promoting the safety and effective...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N21/64
Inventor 安群星穆士杰李翠莹白艳军
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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