Aptamer EpCAM (epithelial cell adhesion molecule) D of EpCAM and preparation method thereof

An adhesion molecule and epithelial cell technology, applied in the field of nucleic acids, can solve the problems of low binding force, unsatisfactory clinical test results, and poor antibody specificity, and achieve the effects of small molecular weight, simple and rapid screening and detection, and good permeability.
CN103387989AActive Publication Date: 2013-11-13XIAMEN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
XIAMEN UNIV
Publication Date
2013-11-13

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Abstract

The invention provides an aptamer EpCAM (epithelial cell adhesion molecule) D of an EpCAM as well as a preparation method and application thereof, relating to nucleic acids. The aptamer has high specificity and affinity. The aptamer has a G tetramer structure or a stem-loop structure. The preparation method comprises the steps of designing and synthesizing a single-stranded DNA (deoxyribonucleic acid) random oligonucleotide bank, screening a target oligonucleotide sequence, and identifying the target protein binding specificity and affinity of the target oligonucleotide sequence by a flow analysis method. The screened aptamer does not have toxicity, has small molecular weight and good permeability, is easy to synthesize and label, only specifically identifies EpCAM proteins and cell lines expressing the EpCAM proteins, and does not have a function of identifying the cell lines which do not express the proteins.
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Description

Technical field

[0001] The present invention relates to a nucleic acid, in particular to a preparation method of a nucleic acid aptamer of EpCAM (Epithelial cell adhesion molecule) epithelial cell adhesion molecule and its application in early detection, treatment and prognosis of tumors. Background technique

[0002] EpCAM (Epithelial cell adhesion molecule) epithelial cell adhesion molecule belongs to the adhesion molecule family, also known as 17-A, ESA, EGP40, Trop-1, KSA, CD326, TACSTD1, CO17-1A, GA733-2 and so on. EpCAM is a single transmembrane protein with a molecular weight of 30-40kDa encoded by tumor-associated calcium signal transducer1 (TACSTD1) gene. It is involved in regulating cell adhesion, mediating signal transduction, and Functions such as migration, proliferation and differentiation (1, Kurtz JE, Dufour P. Adecatumumab: an anti-EpCAM monoclonal antibody, from the bench to the bedside [J]. Expert Opin BiolTher, 2010, 10(6): 951-958; 2. Trzpis M, McLaughlin PM...

Claims

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