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Liquid phase chip used for detecting liver fibrosis and method of producing the same

A liver fibrosis and liquid-phase chip technology, applied in the field of medicine and biology, can solve the problem of inconvenience of clinical diagnosis by detecting only one marker at a time, and achieve the effects of improved detection sensitivity and linear range, less sample size, and high sensitivity

Inactive Publication Date: 2008-05-14
SUREXAM BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] In view of the technical inconvenience of the existing liver fibrosis inspection method that can only detect one marker at a time and the needs of clinical diagnosis, the problem to be solved by the present invention is to provide a liver fibrosis detection liquid chip that can jointly detect four markers, The liquid chip provides a non-invasive, accurate and convenient clinical detection method for the early diagnosis of liver fibrosis

Method used

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  • Liquid phase chip used for detecting liver fibrosis and method of producing the same
  • Liquid phase chip used for detecting liver fibrosis and method of producing the same
  • Liquid phase chip used for detecting liver fibrosis and method of producing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] In the present embodiment, the formula of described various solutions is as follows:

[0049] 1.50mM MES buffer (pH5.0) formula (250ml):

[0050] Reagent

source

Final concentration

Dosage per 250ml

MES

(2[N-Morpholino]

ethanesulfonic

acid)

Sigma M-2933

0.05M

2.44g

5M NaOH

Fisher SS256-500

---

5 drops

[0051] 2. PBS formula:

[0052] Reagent

catalog number

Final concentration

Dosage per 1L

PBS

Sigma-3813

138mM NaCl

2.7mM KCl

1 package

[0053] 3. PBS-TBN formula (that is, PBS containing 0.1% BSA, 0.02% Tween-20, 0.05% Na3N, pH7.4)

[0054] Reagent

catalog number

Final concentration

Dosage per 1L

PBS pH7.4

Sigma P-3813

138mM NaCl

2.7mM KCl

1 package

BSA

Sigma A-9647 ...

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PUM

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Abstract

The invention discloses a liquid phase chip for detecting liver fibrosis, which mainly includes: (1) 4-plex coated microspheres: containing respectively coated type III procollagen (PIIIP) capture antibody, type IV collagen and its fragment (IV-C) capture antibody, laminin (LN) capture antibody and hyaluronidase (HA) capture antibody with different color-coded microspheres; (2) 4-plex biotin-labeled detection antibody: respectively Biotin-labeled detection antibodies of PIIIP, IV-C, LN, HA; and (3) streptavidin phycoerythrin. The invention also discloses a preparation method of the liquid phase chip. The liquid phase chip for liver fibrosis detection of the present invention has the advantages of no side effects, high throughput, multi-indicator parallel detection, high sensitivity, good repeatability, rapid and accurate detection, low cost and the like.

Description

technical field [0001] The invention belongs to the category of medical biology, and in particular relates to a liquid phase chip for detecting liver fibrosis and a preparation method thereof. Background technique [0002] Hepatic fibrosis refers to the continuous and repeated necrosis or inflammatory stimulation of liver cells in various chronic liver diseases, leading to a repair reaction in the body, a large number of fibrous proliferation accompanied by relative or absolute deficiency of fiber degradation, and a large amount of extracellular matrix in the liver. The pathological process of deposition. Liver fibrosis is not an independent disease, but a common pathological process of many chronic liver diseases, and it is also an important reason for the further development and deterioration of chronic hepatitis and cirrhosis. China is a country with a high incidence of viral hepatitis. The main pathological development of various chronic liver diseases is liver cirrhosi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/546
Inventor 许嘉森杨惠夷林一群
Owner SUREXAM BIO TECH
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