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Method for preparing biological polysaccharide self-assembly modificatory chitosan antibacterial biological material

A technology of biological polysaccharides and biological materials, applied in medical science, prosthesis and other directions, can solve the problems such as unreported antibacterial biological materials, and achieve the effects of improving surface biological properties, mild preparation conditions and simple preparation process

Inactive Publication Date: 2010-12-08
WUHAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on the preparation of antibacterial biomaterials by using lentinan and its derivatives to modify the surface of biomaterials.

Method used

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  • Method for preparing biological polysaccharide self-assembly modificatory chitosan antibacterial biological material
  • Method for preparing biological polysaccharide self-assembly modificatory chitosan antibacterial biological material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The preparation method of the chitosan antibacterial biological material of biological polysaccharide self-assembly modification, it comprises the steps:

[0035] 1) Preparation of fungal polysaccharide derivative——lentinan sulfate: add 0.6g lentinan into a reaction bottle containing 50mL dimethyl sulfoxide, stir at 20°C for 12 hours, then slowly add lentinan at a rate of 2mL / min Add 9 mL of pyridine dropwise, continue to stir for 30 minutes, then place the reaction bottle in an ice bath, and slowly add 3.7 mL of chlorosulfonic acid (where chlorosulfonic acid The mol ratio with pyridine is 1: 2), then be warming up to 80 ℃ and continue to stir the reaction for 100 minutes, cool to room temperature after the reaction stops, use the NaOH (concentration is 5wt% to represent that 100gNaOH solution contains NaOH 5g) solution with concentration of 5wt% Adjust the pH to 7.0 to obtain a reaction solution; then inject the obtained reaction solution into a regenerated cellulose d...

Embodiment 2

[0042] The preparation method of the chitosan antibacterial biological material of biological polysaccharide self-assembly modification, it comprises the steps:

[0043] 1) Preparation of fungal polysaccharide derivative-lentinan sulfate: add 1.2g lentinan into a reaction bottle containing 100mL dimethyl sulfoxide, stir at 30°C for 18 hours, and then slowly add lentinan at a rate of 4mL / min. Add 18 mL of pyridine dropwise, continue to stir for 40 minutes, then place the reaction bottle in an ice bath, and slowly add 7.4 mL of chlorosulfonic acid (wherein chlorosulfonic acid and The molar ratio of pyridine is 1: 2), then the temperature is raised to 80°C and the stirring reaction is continued for 120 minutes, after the reaction is stopped, it is cooled to room temperature, and the pH is adjusted to 7.0 with a concentration of 5 wt% NaOH to obtain a reaction solution; then the gained reaction solution is injected into In a regenerated cellulose dialysis bag (36mm, Mw: 8000-14000...

Embodiment 3

[0050] The preparation method of the chitosan antibacterial biological material of biological polysaccharide self-assembly modification, it comprises the steps:

[0051] 1) Preparation of fungal polysaccharide derivative-lentinan sulfate: add 0.6g lentinan into a reaction flask containing 50mL dimethyl sulfoxide, stir at 25°C for 16 hours, and then slowly Add 18 mL of pyridine dropwise, continue to stir for 35 minutes, then place the reaction bottle in an ice bath, slowly add 3.7 mL of chlorosulfonic acid (wherein chlorosulfonic acid and The molar ratio of pyridine is 1: 2), then the temperature is raised to 80°C and the stirring reaction is continued for 120 minutes, after the reaction is stopped, it is cooled to room temperature, and the pH is adjusted to 7.0 with a concentration of 5 wt% NaOH to obtain a reaction solution; then the gained reaction solution is injected into In a regenerated cellulose dialysis bag (36mm, Mw: 8000-14000), dialyze in NaOH solution with pH 10 fo...

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Abstract

The invention relates to a method for preparing a biological polysaccharide self-assembly modificatory chitosan antibacterial biological material, which is characterized by comprising the following steps: (1) preparing a lentinan derivant, i.e. lentinan sulfate; (2) preparing a chitosan membrane base material; (3) preparing a lentinan sulfate solution and a chitosan solution; and (4) carrying outlayer-by-layer self-assembly of the lentinan sulfate and the chitosan on the surface of the base material: respectively carrying out alternative self-assembly on the chitosan membrane base material in the lentinan sulfate solution and the chitosan solution for 5 times to obtain a surface layer-by-layer self-assembly modificatory chitosan mebrane containing 5 bilayers with the outermost layer of the lentinan sulfate, washing and drying to obtain the biological polysaccharide self-assembly modificatory chitosan antibacterial biological material. The chitosan antibacterial biological material prepared by the method has excellent antibacterial activity; and the method has simple preparation process, easy control, mild preparation conditions and low cost.

Description

technical field [0001] The invention belongs to the technical fields of biological materials, polymer chemistry and molecular self-assembly, in particular to a preparation method of chitosan antibacterial biological material modified by biological polysaccharide self-assembly. Background technique [0002] In recent years, biomedical polymer materials have been widely used in the medical field, and more and more polymer materials have been used as substitute materials for human organs and tissues. However, when biomaterials are implanted in the human body, they tend to trigger a series of body defense reactions such as body inflammation and blood coagulation. In addition, the infection of microorganisms such as bacteria during the implantation process can easily lead to postoperative inflammation and other problems, which greatly affect the application of biomedical polymer materials in the medical field. Therefore, it is required that the biomedical polymer material implan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/20A61L27/54C08J7/00C08J7/12C08J5/18C08J3/24C08L5/08C08B37/00
Inventor 王艺峰洪群峰徐敏姚祥雨熊燕飞
Owner WUHAN UNIV OF TECH
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