The invention discloses a preparation method of
polysialic acid-
hyaluronic acid composite gel, an obtained product and an application. The preparation method comprises steps as follows: in an alkaline
aqueous solution environment,
polysialic acid is linked with a crosslinking agent, and activated
polysialic acid is obtained; the activated polysialic acid is added to a
hyaluronic acid solution, so that the polysialic acid is grafted on the
hyaluronic acid in an acid environment, and the product is obtained. The reaction process is controllable, few side reactions exist, the obtained product has excellent
biocompatibility of the hyaluronic acid and non-
immunogenicity of the polysialic acid, can be applied to embedded slow-release of polypeptide or
protein drugs and can also applied to
coating of crosslinked hyaluronic
acid gel particles for injection, the postoperative inflammatory reaction is reduced,
skin redness and swelling are relieved, pain of users is relieved, besides, the product stability can be improved, the degradation time can be prolonged, and the half-life period can be prolonged.